中国病理生理杂志2017，Vol.33Issue(2)：215-220,6.DOI:10.3969/j.issn.1000-4718.2017.02.004
拟交感效应对快速起搏离体心脏心房缝隙连接蛋白43重构的影响
Effects of sympathomimetic agent on remodeling of connexin 43 in atrium of isolated heart with rapid atrial pacing
摘要
Abstract
AIM:To investigate the changes of connexin 43 (Cx43) via establishing a model of sympathomi-metic atrial fibrillation ( AF) .METHODS:The mongrels ( n=15) were randomly divided into control group , rapid atrial pacing (RAP) group and isoprenaline (ISO) perfusion+RAP group (ISO+RAP group).All mongrels’ hearts were taken out rapidly by median sternotomy to establish the cardiac model with Langendorff perfusion in vitro.The atrial effective re-fractory period ( AERP) and AF inducability were tested .The expression and distribution of tyrosine hydroxylase ( TH) were analyzed by immunohistochemistry .Total protein level of Cx 43 and phosphorylation of Cx 43 were determined by West-ern blot.The distribution of Cx43 were also observed by immunofluorescence staining .The cell apoptosis was analyzed by TUNEL staining.The generation of reactive oxygen species ( ROS) in the mitochondria was measured by fluorescence spec-trophotometry .RESULTS:No significant change of AERP was found between control group and RAP group , while that in ISO+RAP group was significantly decreased (P<0.05) and induced AF.Compared with control group, the expression of TH, apoptotic index and the generation of ROS increased gradually (P<0.05), while the content of Cx43 decreased grad-ually both in the total protein and the phosphorylation levels in RAP group and ISO +RAP group (P<0.05).The fluores-cence intensity of Cx43 was also attenuated and Cx43 were lateralized apparently in RAP group , while Cx43 were character-ized as punctate distribution in ISO +RAP group.CONCLUSION:Sympathetic nerves may activate autophagosome at in-tercalated discs and trigger cell apoptosis , resulting in remodeling and downregulation of Cx 43 via oxidative stress , thus having effects on mediating and maintaining AF .关键词
交感神经/缝隙连接蛋白43/心房颤动/氧化应激/自噬Key words
Sympathetic nerves/Connexin 43/Atrial fibrillation/Oxidative stress/Autophagy分类
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舒成霖,何燕,曾志羽,何涛,李金轶,黄伟强,许键,黄艳群..拟交感效应对快速起搏离体心脏心房缝隙连接蛋白43重构的影响[J].中国病理生理杂志,2017,33(2):215-220,6.基金项目
国家自然科学基金资助项目（No．81260039）；广西自然科学基金资助项目（No．2013GXNSFAA278005）；第一批广西医学高层次骨干人才培养“139”计划经费 （）
