中国病理生理杂志2017，Vol.33Issue(2)：271-277,7.DOI:10.3969/j.issn.1000-4718.2017.02.013
姜黄素通过下调 nectin-4抑制肺癌PC-9细胞的迁移和侵袭
Curcumin inhibits migration and invasion of lung cancer PC-9 cells via down-regulation of nectin-4 expression
摘要
Abstract
AIM:To investigate the effects of curcumin on the abilities of migration and invasion in the lung cancer PC-9 cells, and to observe the relationship between curcumin and nectin-4 expression.METHODS:The viability, migration and invasion of lung cancer PC-9 cells treated with curcumin or transfected with siNectin-4 were measured by MTT assay, wound healing test and Transwell assay , respectively.The protein levels of nectin-4, p-AKT and AKT in the PC-9 cells treated with curcumin or transfected with siNectin-4 were detected by Western blot .RESULTS:Curcumin in-hibited the viability of PC-9 cells.The wound healing rates and the numbers of the transmembrane cells in curcumin 10μmol/L and 20 μmol/L groups were decreased compared with control group without curcumin treatment .The expression level of nectin-4 was reduced after curcumin treatment for 24 h.The viability of the PC-9 cells was significantly inhibited after transfected with siNectin-4 for 48 h or 72 h (P<0.01), and the wound healing rates was decreased in siNectin-4 group compared with NC group (P<0.01).The numbers of the transmembrane cells in siNectin-4 group was significantly reduced (P<0.01).Curcumin and knockdown of nectin-4 suppressed the activation of AKT pathway in PC-9 cells.In si-Nectin-4+curcumin group , the cell viability reduced compared with curcumin group , and wound healing rates , cell inva-sive ability and AKT phosphorylation levels were decreased .CONCLUSION:Curcumin inhibits migration and invasion of the lung cancer PC-9 cells via down-regulation of nectin-4 expression and inhibition of AKT pathway .关键词
姜黄素/肺癌/细胞迁移/细胞侵袭/Nectin-4Key words
Curcumin/Lung cancer/Cell migration/Cell invasion/Nectin-4分类
医药卫生引用本文复制引用
陈君,焦德敏,唐夏莉,王剑,李优,陈清勇..姜黄素通过下调 nectin-4抑制肺癌PC-9细胞的迁移和侵袭[J].中国病理生理杂志,2017,33(2):271-277,7.基金项目
浙江省医药卫生科技计划（No．2011KYA139）；浙江省科技厅公益性技术应用研究计划（No．2014C33277）；杭州市科技发展计划（No．20130633B29；No．20140633B40）；浙江省自然科学基金资助项目 （）
