中国病理生理杂志2017，Vol.33Issue(2)：284-288,5.DOI:10.3969/j.issn.1000-4718.2017.02.015
低表达miR-21对苦参碱诱导的肝癌HepG2细胞凋亡的影响
Effect of low expression of miR-21 on apoptosis of HepG2 cells induced by matrine
摘要
Abstract
AIM:To explore whether miR-21 low expression enhances the effect of matrine ( MAT) on the ap-optosis of hepatocellular carcinoma cells .METHODS:Real-time fluorescence quantitative PCR ( RT-qPCR) was used to detect the expression of miR-21 in the HepG2 cells treated with different concentrations of MAT .The effect of miR-21 on MAT-induced HepG2 cell apoptosis was analyzed by flow cytometry .The mRNA and protein expression of Bcl-2 and Bax in the HepG2 cells treated with MAT was determined by RT-qPCR and Western blot .RESULTS:The expression of miR-21 increased with the increasing concentration of MAT .Low expression of miR-21 promoted MAT-induced apoptosis , and en-hanced the expression of Bax at mRNA and protein levels ( P<0.05 ) , while inhibited the expression of Bcl-2 at mRNA and protein levels (P<0.05).CONCLUSION:Low expression of miR-21 enhances MAT-induced HepG2 cell apoptosis by inhibiting the expression of Bcl-2 and promoting Bax expression .关键词
miR-21/苦参碱/HepG2细胞/细胞凋亡Key words
miR-21/Matrine/HepG2 cells/Apoptosis分类
医药卫生引用本文复制引用
罗耀玲,黄铀新,赖平,张敏鸿..低表达miR-21对苦参碱诱导的肝癌HepG2细胞凋亡的影响[J].中国病理生理杂志,2017,33(2):284-288,5.基金项目
赣南医学院校级课题 （）
