中国病理生理杂志2017，Vol.33Issue(2)：308-314,7.DOI:10.3969/j.issn.1000-4718.2017.02.019
白蛋白过载加重阿霉素肾病小鼠肾损害及辛伐他汀的肾脏保护作用
Albumin overload aggravates kidney injury and renoprotective effect of simvastatin in adriamycin nephropathy mice
摘要
Abstract
[ ABSTRACT] AIM:To investigate the aggravating effect of albumin overload on the kidney injury induced by lip -id nephrotoxicity , and to observe the renoprotective effect of simvastatin ( SIMV) on adriamycin nephropathy ( ADR) mice. METHODS:SPF healthy male BALB/c mice were randomly divided into control group , ADR group, ADR with bovine se-rum albumin ( BSA) overload ( ADR+BSA) group, and ADR with both BSA overload and SIMV treatment ( ADR+BSA+SIMV) group.All mice were uninephrectomized under general anesthesia 2 weeks before setting up ADR model .ADR+BSA model started to be set up 4 weeks later.At the end of the 0th, 2nd, 6th, 10th and 14th weeks, 24 h urinary protein was evaluated.At the end of the 14th week, the serum biochemical indexes and the kidney pathological changes were ob -served, and glomerulosclerotic index (GSI) was also evaluated.The cholesterol in the kidney was measured by enzymic colorimetric method and oil red O staining .The expression of IL-1β, TGF-β1 and low-density lipoprotein receptor ( LDLr) in the kidney tissues was determined by real-time PCR.The expression of IL-1βand IL-17 was measured by immunohisto-chemistry and the expression of IL-17 in the kidney was measured by ELISA .RESULTS: Compared with control group , the expression of IL-1β, TGF-β1, IL-17 and LDLr, and cholesterol content in the kidney and the GSI were all significantly increased in ADR group (P<0.05).Compared with ADR group, 24 h urinary protein, serum creatinine, the expression of IL-1β, IL-17 and LDLr, and cholesterol content in the kidney were all significantly increased in ADR +BSA group ( P<0.05).Treatment with SIMV significantly decreased the expression of IL-1β, TGF-β1, IL-17 and LDLr.The accumulation of cholesterol in the kidney and the GSI were also decreased (all P<0.05).CONCLUSION: Inflammation aggravates the lipid deposition and glomerular sclerosis by increasing the expression of LDLr in ADR mice .Albumin overload further accelerates the progressive kidney damage by regulating the expression of IL-1β, TGF-β1 and IL-17, which promotes the increase in LDLr .The beneficial effect of SIMV might be mediated by its anti-inflammatory effect .关键词
蛋白尿/炎症/阿霉素肾病/低密度脂蛋白受体/脂质/辛伐他汀Key words
Proteinuria/Inflammation/Adriamycin nephropathy/Low-density lipoprotein receptor/Lipid/Simvastatin分类
医药卫生引用本文复制引用
李瑶,魏心怡,闫俊丽,王墨,吴道奇,张高福,阳海平,李秋..白蛋白过载加重阿霉素肾病小鼠肾损害及辛伐他汀的肾脏保护作用[J].中国病理生理杂志,2017,33(2):308-314,7.基金项目
国家自然科学基金资助项目（No．81270802）；国家自然科学基金青年科学基金资助项目 （）
