中国病理生理杂志2017，Vol.33Issue(2)：380-384,5.DOI:10.3969/j.issn.1000-4718.2017.02.032
动态观察过敏原诱导的哮喘小鼠模型从急性炎症到慢性重塑的发展
Dynamic observation of asthma model induced by allergen in mice from acute inflammation to chronic remodeling
摘要
Abstract
AIM:To dynamically observe and compare the relative changes of the indexes from the process of acute inflammation to chronic remodeling in asthmatic mice induced by ovalbumin ( OVA) .METHODS:Female BALB/c mice (n=60) were randomly divided into normal control group and asthma group .The mice in asthma group were sensi-tized and challenged by OVA , while the mice in normal group received equal volume of normal saline ( NS) .The challenge was performed for 3 consecutive days from the 21th day to observe the response of acute inflammation , and then the mice in different groups were challenged once per week for 5 weeks.Detailed comparisons of the dynamic changes of cell infiltra-tion, cytokine expression and airway remodeling were conducted .RESULTS:Compared with NS group , the mice in OVA group showed a predominantly eosinophilic infiltration into the airway lumen , increased production of Th 2-type cytokines , secretion of epithelial mucus and deposition of subepithelial collagen .In OVA challenge groups , the levels of inflammatory cells and inflammatory factors were remarkably higher in 24 d group, whereas the most obvious changes of goblet cell hyper-plasia and airway remodeling were observed in 52 d group.CONCLUSION:Acute asthma model is sufficiently induced by 3 consecutive days of OVA challenge protocol , which is accompanied with high levels of inflammatory cells and inflammato-ry factors.The OVA challenge protocol once per week for 5 weeks could induce a chronic asthma model with obvious airway remodeling .关键词
过敏原/气道炎症/气道重塑/哮喘模型Key words
Allergen/Airway inflammation/Airway remodeling/Asthma model分类
医药卫生引用本文复制引用
张方琪,韩新鹏,张芳,杨学敏,向东,李志奎..动态观察过敏原诱导的哮喘小鼠模型从急性炎症到慢性重塑的发展[J].中国病理生理杂志,2017,33(2):380-384,5.基金项目
国家自然科学基金资助项目 （）
