中国感染控制杂志2017，Vol.16Issue(2)：99-103,5.DOI:10.3969/j.issn.1671-9638.2017.02.001
重症监护病房分离金黄色葡萄球菌的分子流行特征
Molecular epidemiological characteristics of Staphylococcus aureus isola-ted in intensive care units
摘要
Abstract
Objective To investigate the molecular epidemiological characteristics and homology of Staphylococcus aureus (S. aureus)isolated from patients in intensive care units (ICUs)of a hospital,so as to provide laboratory basis for the effective control of healthcare-associated infection(HAI). Methods 62 S. aureus strains isolated from various specimens from ICU patients with infection in March-August 2013 were collected,7 housekeeping genes were amplified with polymerase chain reaction (PCR),the amplified products were sequenced,ST typing of strains was performed by multilocus sequence typing (MLST ), phylogenetic analysis of ST typing was conducted. Results 62S. aureus strains were amplified specific product of 7 housekeeping genes;there were 10 ST genotypes, in which 2 ST genotypes(STn1and STn2)were first discovered,1 ST genotype(ST675)was first discovered domes-tically. ST239 was the main ST type of S. aureus from ICU patients in this hospital,accounting for 74.20% ,which distributed in 6 ICUs,ST5 distributed in 3 ICUs. 62 strains formed 7 main branches in the phylogenetic tree,55 (88.71% )MRSA strains were detected. Conclusion S. aureus isolated from hospital ICUs has some homology, and the small number of types showed the trend of concentrated distribution.关键词
重症监护病房/金黄色葡萄球菌/多位点序列分型/基因/同源性Key words
intensive care unit/Staphylococcus aureus/multilocus sequence typing/gene/homology分类
医药卫生引用本文复制引用
杨玉琪,徐修礼,马越云,史皆然,周珊,周磊,郝晓柯..重症监护病房分离金黄色葡萄球菌的分子流行特征[J].中国感染控制杂志,2017,16(2):99-103,5.基金项目
陕西省自然科学基金资助项目(2014JM4188) （2014JM4188）
国家仪器重大专项(2012YQ03026107) （2012YQ03026107）
