中国感染控制杂志2017，Vol.16Issue(2)：104-108,5.DOI:10.3969/j.issn.1671-9638.2017.02.002
徐州地区116株MRSA耐药性分析与分子流行病学调查
Antimicrobial resistance and molecular epidemiology of 116 strains of me-thicillin-resistant Staphylococcus aureu in Xuzhou area
摘要
Abstract
Objective To investigate infection status and antimicrobial resistance mechanism of methicillin-resistant Staphylococcusaureus(MRSA),and provide reference for the rational antimicrobial use in clinic. Methods Staphylococcusaureus (SA)isolated from various specimens in Xuzhou area in 2012-2015 were collected,MR-SA strains were preliminarily screened by cefoxitin disk diffusion method,and confirmed by amplification of mecA gene,antimicrobial resistance of MRSA was determined by Kirby-Bauer method,minimal inhibitory concentration (MIC)was measured by E-test method,genotypes of staphylococcal chromosomal cassette mec(SCCmec)were de-termined by multiplex PCR. Results A total of 116 strains of MRSA were identified among 210 SA strains in 2012-2015,114 of which were positive for mecA gene,the total detection rate of MRSA was 55.24% . Susceptibility rates of MRSA to vancomycin,quinupristin/dalfopristin,and linezolid were all 100% ,resistance rates of MRSA to chloramphenicol and furantoin were both low,which were 15.52% and 1.72% respectively,resistance rates of MR-SA to 10 kinds of antimicrobial agents were all>80% ;resistance rates of MRSA to penicillins,aminoglycosides, macrolides,quinolones,sulfanilamide,rifampicin,tetracycline,and clindamycin were all higher than methicillin-sensitive Staphylococcusaureus(MSSA). MICs of vancomycin to MRSA in 2012-2015 were all 1.0μg/mL,MIC90 were all 1.5μg/mL,one MRSA isolate was with a vancomycin MIC of 2.0μg/mL in 2015. MRSA typing results of 116 MRSA isolates showed that SCCmec II,SCCmec III,and SCCmec IV accounted for 9.48% (n= 11),73.28% (n= 85),and 1.72% (Iva,n= 2;IVb,n= 2)respectively,13.79% (n= 16)of MRSA isolates were nontypeable, SCCmec I and SCCmec V type strains were not found. Conclusion MRSA is seriously multidrug-resistant,the drift has not been discovered in MIC value of vancomycin against MRSA,the major SCCmec genotype of MRSA is SCCmec III,infection control measures should be taken to control MRSA infection.关键词
耐甲氧西林金黄色葡萄球菌/MRSA/耐药性/最低抑菌浓度/MIC/葡萄球菌染色体mec盒/SCCmecKey words
methicillin-resistant Staphylococcus aureus/MRSA/drug resistance/minimal inhibitory concentra-tion/MIC/staphylococcal chromosomal cassette mec/SCCmec分类
医药卫生引用本文复制引用
纵帅,徐萍萍,顾兵,郝婷婷,寇艳波,徐银海..徐州地区116株MRSA耐药性分析与分子流行病学调查[J].中国感染控制杂志,2017,16(2):104-108,5.基金项目
徐州市科技局资助项目(KC14SH116) （KC14SH116）
