中国感染控制杂志2017，Vol.16Issue(2)：142-145,4.DOI:10.3969/j.issn.1671-9638.2017.02.010
新生儿、医务人员及环境分离耐甲氧西林金黄色葡萄球菌的同源性
Homology of methicillin-resistant Stphylococcusaureus isolated from neo-nates,health care workers,and environment
摘要
Abstract
Objective To investigate the homology of methicillin-resistant Stphylococcusaureus(MRSA)from the neonatal intensive care unit(NICU)of a children's hospital,and evaluate routes and preventive strategies of MRSA healthcare-associated infection(HAI). Methods MRSA strains from neonates and environment of NICU between October and December 2014 were collected,and strains were identified by VITEK-2 microbial analysis system and cefoxitin Kirby-Bauer method,homology of MRSA was analyzed by pulsed-field gel electrophoresis (PFGE ). Results A total of 6 MRSA strains were isolated from NICU between October and December 2014,3 of which (bed-58,70,and 100)were detected MRSA from specimens,MRSA were isolated from neonatal incubator and nurse (nasal swabs and hands)who cared for neonate at bed 58. 5 of 6 MRSA strains were homology,antimicrobial susceptibility testing result showed that No. 1-5 strains were resistant to clindamycin and amoxicillin/clavulanic acid,No. 6 strain was slightly different from No. 1-5 strains,No. 6 strain was susceptible to both clindamycin and amoxicillin/clavulanic acid. PFGE results showed that No. 1-5 strains were of the same type,No. 6 strain was a different type. Conclusion The main route of this MRSA transmission is contact transmission,especially through the hands of health care workers,identification and analysis of epidemic strains by PFGE technique is an effective measures to prevent HAI outbreak and perform epidemiological study.关键词
新生儿/新生儿重症监护病房/NICU/金黄色葡萄球菌/医院感染/脉冲场凝胶电泳/流行病学Key words
neonate/neonatal intensive care unit/NICU/Staphylococcusaureus/healthcare-associated infection/pulsed-field gel electrophoresis/epidemiology分类
医药卫生引用本文复制引用
胡瑛,文飞球,刘犇,钟春梅,付雪梅..新生儿、医务人员及环境分离耐甲氧西林金黄色葡萄球菌的同源性[J].中国感染控制杂志,2017,16(2):142-145,4.基金项目
2014 年深圳市科技计划项目(JCYJ20140416141331496) （JCYJ20140416141331496）
