中国康复理论与实践2017，Vol.23Issue(1)：27-31,5.DOI:10.3969/j.issn.1006-9771.2017.01.007
针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响
Effects of Acupuncture-rehabilitation Therapy on Neurological Function and Extracellular Signal-regulated Ki-nase 1/2 Signaling Pathway after Focal Cerebral Ischemia in Rats
摘要
Abstract
Objective To explore the effects of acupuncture-rehabilitation therapy on neurological function recovery and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway activation after permanent focal cerebral ischemia in rats. Methods Ninty male Sprague-Dawley rats were divided into five groups, namely sham group, model group, acupuncture group, rehabilitation group and acupunc-ture-rehabilitation group, and 18 in each group. Each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Their mid-dle cerebral arteries were occluded except those of sham group. The sham and model groups accepted no treatment, while the acupuncture group accepted cluster needling of scalp acupuncture, rehabilitation group accepted treadmill training, and the acupuncture-rehabilitation group accepted both acupuncture and treadmill training. They were assessed with modified Neurologic Severity Score (mNSS) 3, 7 and 14 days after modeling, while the expression of ERK1/2 and p-ERK1/2 were determined with Western blotting. Results The neurologic severi-ty score reduced in all three treatment groups (P<0.05) compared with that of the model group at every time point, and was the least in the acupuncture-rehabilitation group (P<0.05) 7 and 14 days after modeling among the treatment groups. Meanwhile, the expression of p-ERK1/2 increased in all three treatment groups (P<0.05), and was the most in the acupuncture-rehabilitation group (P<0.05), as well as the rate of (p- ERK1/2)/(ERK1/2) (P<0.05). Conclusion Acupuncture- rehabilitation therapy can promote the neurological function recovery, which may be associated with activation of ERK1/2 signaling pathway.关键词
脑缺血/针康法/神经功能/细胞外信号调节激酶1/2/大鼠Key words
cerebral ischemia/acupuncture-rehabilitation therapy/neurological function/extracellular signal-regulated kinase 1/2/rats分类
医药卫生引用本文复制引用
唐强,叶涛,朱路文,吴孝军,李宏玉,田源..针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响[J].中国康复理论与实践,2017,23(1):27-31,5.基金项目
1.国家自然科学基金项目(No.81273818 （）
No.81473762) （）
2.黑龙江中医药大学领军人才计划项目(No.2012RCL02). （No.2012RCL02）
