中国康复理论与实践2017，Vol.23Issue(1)：37-41,5.DOI:10.3969/j.issn.1006-9771.2017.01.09
双光子显微镜在小动物活体光学成像中的研究进展
Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)
摘要
Abstract
Two-photon microscopy is a new technique which combines laser scanning con-focal microscopy and two-photon excitation technique. Two-photon fluorescence microscopy has the advantages of little light damage, small bleaching area, strong penetrability, high resolution, high fluorescence collection efficiency, and high image contrast. It is suitable for dark field imaging and multi-labeled compound measurement, and has been widely used in small animals in vivo optical imaging, such as research for tumour, gene therapy, stem cells, drug development, spinal cord injury, etc.关键词
双光子显微镜/活体光学成像/小动物/综述Key words
two-photon microscopy/in vivo optical imaging/small animals/review分类
医药卫生引用本文复制引用
张文豪,李建军,杨德刚,杨明亮,杜良杰,高峰,刘长彬,李大鹏,胡安明..双光子显微镜在小动物活体光学成像中的研究进展[J].中国康复理论与实践,2017,23(1):37-41,5.基金项目
1.国家自然科学基金项目(No.81272164) （No.81272164）
2.中央级公益性科研院所基本科研业务费专项资金(No.2015CZ-6) （No.2015CZ-6）
3.中国康复研究中心课题(No.2012-1 （）
No.2013-7). （）
