中国康复理论与实践2017，Vol.23Issue(2)：155-161,7.DOI:10.3969/j.issn.1006-9771.2017.02.008
神经营养因子3-壳聚糖载体对大鼠运动皮层损伤后内源性神经发生和运动功能的效果
Effect of Neurotrophin 3-chitosan on Endogenous Neurogenesis and Motor Function after Motor Cortex Injury in Rats
摘要
Abstract
Objective To observe the effects of neurotrophin 3 (NT3)-chitosan on motor function, and proliferation and differentiation of the neural stem cells (NSCs) in the injury area and subventricular zone (SVZ) in rats with motor cortex injury. Methods Sixty-five Wistar rats were divided into control group (n=7), injury group (n=29) and NT3-chitosan group (n=29). The motor cortex was aspirated and re-moved as cerebral injury model. NT3-chitosan was immediately implanted into the injured area after operation, and the control group re-ceived no intervention. Pellet reaching test was performed to detect the recovery of the forelimb function, HE staining was used to observe the lesion cavity size, and immunofluorescence staining was used to observe the proliferation and differentiation of NSCs 3 days, 7 days, 14 days, 1 month, 2 months and 3 months after operation. Results The grasp success rate was higher (F>6.00, P≤0.05), and the lesion cavity size was significantly smaller (F>629.5, P<0.001) in the NT3-chitosan group than in the injury group. In the NSCs differentiation experi-ment, the number of BrdU cells at all the time points was significantly higher in the NT3-chitosan group than in the injury group (F>171.43, P<0.001). In the NSCs proliferation experiment, the number of BrdU positive cells was still significantly higher in the NT3-chitosan group than in the control group and in the injury group (F>155.06, P<0.001), the number of Dcx positive cells was significantly higher in the NT3-chitosan group than in the injury group (F=62.367, P<0.001), and the number of BrdU/Dcx positive cells was significantly higher in the NT3-chitosan group than in the control group (F=33.527, P<0.001). Conclusion NT3-chitosan could activate NSCs in the SVZ, and pro-mote endogenous neurogenesis and forelimb function recovery in rats after motor cortex injury.关键词
创伤性脑损伤/神经营养因子3/壳聚糖/内源性神经发生/运动功能/大鼠Key words
traumatic brain injury/neurotrophin 3/chitosan/endogenous neurogenesis/motor function/rats分类
医药卫生引用本文复制引用
杨飞祥,张皑峰,郝鹏,尚俊奎,段红梅,杨朝阳,李晓光..神经营养因子3-壳聚糖载体对大鼠运动皮层损伤后内源性神经发生和运动功能的效果[J].中国康复理论与实践,2017,23(2):155-161,7.基金项目
1.国家自然科学基金重点项目(No.31130022) （No.31130022）
2.国家自然科学基金国际(地区)合作与交流项目(No.31320103903) （地区）
3.国家自然科学基金面上项目(No.31271037 （）
No.31670988) （）
4.国家自然科学基金应急管理项目(No.31650001) （No.31650001）
5.高等学校全国优秀博士学位论文作者专项资金资助项目(No.201356). （No.201356）
