中国农业科学2017，Vol.50Issue(1)：123-130,8.DOI:10.3864/j.issn.0578-1752.2017.01.011
六倍体(AnAnCnCnCoCo)与白菜型油菜杂交可交配性及后代菌核病抗性
Crossability and Sclerotinia Resistance Among Hybrids Between Hexaploid (AnAnCnCnCoCo) and Brassica rapa
摘要
Abstract
[Objective] Utilization of parental species is helpful to broaden and improve genetic basis of Brassica napus. A strategy of using hexaploid derived from hybrid between B. napus and B. oleracea as bridge was evaluated to improve B. napus by crossing with B. rapa. [Method] Hexaploid derived from interspecific hybrid between B. napus (Zhongshuang 9) and B. oleracea (C01, high resistant to Sclerotinia sclerotiorum) was developed to cross with B. rapa by evaluating its fertility, meiotic chromosome segregation and resistance to S. sclerotiorum. Hybrids between hexaploid and 110 B. rapa were developed to evaluate crossability, seed setting percentage and resistance to S. sclerotiorum. [Result]Hexaploid had higher resistance to S. sclerotiorum than Zhongshuang 9. Its pollen fertility was 90.6%-92.7%, seed setting was 3-7 seeds/pod. The 68.80% pollen mother cells at anaphase I was 28﹕28. The hybrids between 110 B. rapa and hexaploid could be obtained when hexaploid was used as either female or male parent, with an average seed setting rate of (4.25±3.91) seeds/pod (hexaploid used as female parent: 4.27 seeds/pod, male: 3.95 seeds/pod on average, P=0.69). Embryos 15 d after pollination grew well when crossing hexaploid with B. rapa. Although crossability among genotypes of B. rapa with hexaploid was different, no significant difference was detected in crossability among ecotypes of B. rapa (semi-winter: (4.35±3.77) seeds/pod, spring: (4.34±4.51) seeds/pod, winter: (4.01±3.43) seeds/pod,P=0.44). Hybrids owned kinds of morphology at the seedling stage, but were similar with B. napus. Seed setting rate of hybrids was (7.72± 4.45) seeds/pod on an average, without significant difference among hybrids derived from spring, winter and semi-winter ecotypes of B. rapa (seed setting rate of hybrids derived from winter: (8.07±3.43) seeds/pod, semi-winter: (7.88±4.64) seeds/pod, spring:(6.41±3.00) seeds/pod, P=0.95). Compared with Zhongshuang 9, 6 hybrids had higher resistance to S. sclerotiorum via two years identification (P<0.05). [Conclusion]It was concluded that it is an efficient way to improve B. napus by transferring elite traits from parental species via hexaploid strategy.关键词
六倍体/甘蓝型油菜/白菜型油菜/可交配性/菌核病Key words
hexaploid/Brassica napus/B. rapa/crossability/Sclerotinia sclerotiorum引用本文复制引用
李勤菲,陈致富,刘瑶,梅家琴,钱伟..六倍体(AnAnCnCnCoCo)与白菜型油菜杂交可交配性及后代菌核病抗性[J].中国农业科学,2017,50(1):123-130,8.基金项目
中国博士后科学基金(2015M582500)、中央高校基本科研业务费专项(XDJK2016C080) （2015M582500）
