中国药房2017，Vol.28Issue(6)：809-812,4.DOI:10.6039/j.issn.1001-0408.2017.06.24
RP-HPLC法测定新扶正除疫颗粒中红景天苷和虎杖苷的含量
Contents Determination of Salidroside and Polydatin in New Fuzheng Chuyi Granule by RP-HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of salidroside and polydatin in new Fuzheng chuyi granule. METHODS:RH-HPLC was performed on the column of GRACE Alltima TMC 18 with mobile phase of methanol-water(15:85,V/V,salidroside)and acetonitrile-water(23:77,V/V,polydatin)at a flow rate of 1.0 mL/min,detection wavelength was 275 nm (salidroside) and 306 nm (polydatin),column temperature was 25 ℃,and the injection volume was 10 μL. RE-SULTS:The linear range was 0.99-10.89 μg for salidroside(r=0.9998)and 0.046-1.196 μg for polydatin(r=0.9998);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 96.31%-99.44%(RSD=1.20%,n=6) and 95.96%-99.73%(RSD=1.42%,n=6). CONCLUSIONS:The method is simple,accurate and reliable,and suitable for the con-tents determination of salidroside and polydatin in new Fuzheng chuyi granule.关键词
反相高效液相色谱法/新扶正除疫颗粒/红景天苷/虎杖苷/含量测定Key words
RP-HPLC/New Fuzheng chuyi granule/Salidroside/Polydatin/Content determination分类
医药卫生引用本文复制引用
付美丽,严铭铭,邵帅,吴程彦,刘畅,田双,尉忠贤,杨洋..RP-HPLC法测定新扶正除疫颗粒中红景天苷和虎杖苷的含量[J].中国药房,2017,28(6):809-812,4.基金项目
国家科技重大专项课题(No.2012ZX09103201-033) （No.2012ZX09103201-033）
吉林省科技发展计划项目(No.20150204036YY) （No.20150204036YY）
