中国药房2017，Vol.28Issue(6)：812-815,4.DOI:10.6039/j.issn.1001-0408.2017.06.25
RP-HPLC法同时测定桑杏口服液中芦丁、橙皮苷、牛蒡苷和甘草苷的含量
Simultaneous Determination of Rutin, Hesperidin,Arctiin and Liquiritin in Sangxing Oral Liquid by RP-HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of rutin ,hesperidin ,arctiin ,liquiritin and glycyrrhizinate in Sangxing oral liquid. METHODS:RP-HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.1% phosphoric acid solution(gradient elution) at a flow rate of 1.0 mL/min,the detection wavelength was 276, 348 nm ,column temperature was 30 ℃,and the injection volume was 5 μL. RESULTS:The linear range was 1.068-10.68 μg/mL (r=0.9993)for rutin,9.320-93.20 μg/mL(r=0.9992)for hesperidin,46.91-469.1 μg/mL(r=0.9994)for arctiin,0.5110-5.110μg/mL(r=0.9992) for liquiritin;RSDs of precision , stability and reproducibility tests were less than 3%;recoveries were 95.10% -101.90%(RSD=2.1%,n=9),96.75%-101.80%(RSD=1.4%,n=9),95.69%-100.00%(RSD=1.5%,n=9), 98.22%-104.60%(RSD=2.1%,n=9),respectively. CONCLUSIONS:The method is accurate,sensitive and reproducible,and can be used for the simultaneous determination of rutin,hesperidin,arctiin and liquiritin in Sangxing oral liquid.关键词
桑杏口服液/反相高效液相色谱法/芦丁/橙皮苷/牛蒡苷/甘草苷Key words
Sangxing oral liquid/RP-HPLC/Rutin/Hesperidin/Arctiin/Liquiritin分类
医药卫生引用本文复制引用
程雪,谈瑄忠,苏联麟,陆兔林,毛春芹,张雪荣..RP-HPLC法同时测定桑杏口服液中芦丁、橙皮苷、牛蒡苷和甘草苷的含量[J].中国药房,2017,28(6):812-815,4.基金项目
国家临床重点专科建设项目(No.苏中医综〔2014〕3号) （No.苏中医综〔2014〕3号）
南京市科技发展计划项目(No.201605035) （No.201605035）
