中国药房2017，Vol.28Issue(6)：823-826,4.DOI:10.6039/j.issn.1001-0408.2017.06.28
蒙药益肾散的质量标准研究
Study on the Quality Standard for Mongolian Medicine Yishen Powder
摘要
Abstract
OBJECTIVE:To establish the quality standard for Mongolian medicine Yishen powder. METHODS:TLC was used for the qualitative identification of Rheum palmatum and Terminalia chebula in the preparation;HPLC was used for the contents de-termination of aloe emodin,rhein,emodin,chrysophanol and physcion:the column was Inertsil C18 with mobile phase of metha-nol-0.1% phosphoric acid(gradient elution)at a flow rate of 1.0 mL/min,detection wavelength was 254 nm,column temperature was 35 ℃ and the injection volume was 10 μL. RESULTS:The TLC pots of R. palmatum and T. chebula were clear and well-sepa-rated,negative control without interference. The linear range was 23.55-117.75 ng for aloe emodin(r=0.9999),44.72-223.62 ng for rhein(r=0.9998),43.18-215.90 ng for emodin(r=0.9997),77.41-387.12 ng for chrysophanol(r=0.9999)and 46.02-230.10 ng for physcion (r=0.9997);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 95.80%-99.66%(RSD=1.21%,n=6),95.01%-98.07%(RSD=0.92%,n=6),95.06%-97.84%(RSD=0.5%,n=6),95.19%-97.66%(RSD=1.07%,n=6)and 95.07%-98.20%(RSD=0.95%,n=6). CONCLUSIONS:The established standard can be used for the quality control of Mongolian medicine Yishen powder.关键词
蒙药益肾散/薄层色谱法/高效液相色谱法/含量测定Key words
Mongolian medicine Yishen powder/TLC/HPLC/Content determination分类
医药卫生引用本文复制引用
王美丽,海祺山,戴丽丽,田香,白玉霞..蒙药益肾散的质量标准研究[J].中国药房,2017,28(6):823-826,4.基金项目
通辽市与内蒙古民族大学科技合作项目(No. SXZD2012011) （No. SXZD2012011）
