中国药房2017，Vol.28Issue(6)：835-837,3.DOI:10.6039/j.issn.1001-0408.2017.06.31
改进HPLC法同时测定注射用替卡西林钠克拉维酸钾中两种主成分的含量
Simultaneous Determination of 2 Main Components in Ticarcillin Disodium and Potassium Clavulanate for Injection by Improved HPLC
王茉莉 1张菁 2张少萌 1张兰桐1
作者信息
- 1. 河北省药品检验研究院,石家庄 050011
- 2. 河北医科大学药学院,石家庄 050017
- 折叠
摘要
Abstract
OBJECTIVE:To improve the method for the determination of 2 main components in Ticarcillin disodium and potas-sium clavulanate for injection. METHODS:HPLC was performed on the column of Waters XBridgeTM C18 with mobile phase of 0.01 mol/L ammonium dibasic phosphate solution(pH 7.0)-menthol(80:20,V/V)at a flow rate of 1.0 mL/min,the detection wave-length was 220 nm, column temperature was 30 ℃, and injection volume was 20 μL. RESULTS:The linear range was 1.95-195.22 μg/mL for ticarcillin (r=0.9999) and 0.12-12.18 μg/mL for clavulanate(r=0.9999);RSDs of precision,stability (under 4 ℃) and reproducibility tests were lower than 1.0%;recoveries were 99.3%-100.5%(RSD=0.4%,n=9) and 99.2%-101.0%(RSD=0.7%,n=9). CONCLUSIONS:The method is rapid,accurate and reliable,and can be used for the determination of 2 main components in Ticarcillin disodium and potassium clavulanate for injection.关键词
高效液相色谱法/替卡西林/克拉维酸/注射用替卡西林钠克拉维酸钾/含量测定Key words
HPLC/Ticarcillin/Clavulanate/Ticarcillin disodium and potassium clavulanate for injection/Content determina-tion分类
医药卫生引用本文复制引用
王茉莉,张菁,张少萌,张兰桐..改进HPLC法同时测定注射用替卡西林钠克拉维酸钾中两种主成分的含量[J].中国药房,2017,28(6):835-837,3.
