中国药房2017，Vol.28Issue(6)：838-841,4.DOI:10.6039/j.issn.1001-0408.2017.06.32
抗骨增生片的质量标准提高研究
Study on Improving the Quality Standards of Kanggu Zengsheng Tablet
陈希 1万林春 1张文婷1
作者信息
- 1. 江西省药品检验检测研究院/江西省药品与医疗器械质量工程技术研究中心,南昌330029
- 折叠
摘要
Abstract
OBJECTIVE:To develop and improve the quality standard for Kanggu zengsheng tablet. METHODS:TLC was used for the qualitative identification of Drynaria fortunei,Epimedii Folium and Spatholobus suberectus;HPLC was used for the contents determination of icariin and acteoside:the column was Diamonsil C18 with mobile phase of acetonitrile-0.1% formic acid solution (gradient elution) at a flow rate of 1.0 mL/min;detection wavelength was 270 nm for icariin and 334 nm for acteoside, Cdumn temperature was 25 ℃,and the injection volume was 10 μL. RESULTS:The TLC spots of D. fortunei,Epimedii Folium and S. suberectus were clear and well separated,negative control without interference. The linear range was 0.0188-1.88 μg for ac-teoside(r=0.9999)and 0.107-2.14 μg for icariin(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 100.2%-105.0%(RSD=1.6%,n=9) and 96.2%-99.5%(RSD=1.4%,n=9). CONCLUSIONS:The improved standard can more effectively control the quality of Kanggu zengsheng tablet.关键词
抗骨增生片/柚皮苷/淫羊藿苷/毛蕊花糖苷/鸡血藤/薄层色谱法/高效液相色谱法Key words
Kanggu zengsheng tablet/Naringin/Icariin/Acteoside/Spatholobus suberectus/TLC/HPLC分类
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陈希,万林春,张文婷..抗骨增生片的质量标准提高研究[J].中国药房,2017,28(6):838-841,4.
