中国医学前沿杂志（电子版）2017，Vol.9Issue(1)：73-77,5.DOI:10.12037/YXQY.2017.01-12
阿司匹林影响人牙髓间充质干细胞成骨分化的新型药理研究
The effect of aspirin in regulating the osteogenesis of human dental pulp derived mesenchymal stem cells
摘要
Abstract
Objective To study the effect of aspirin in regulating the osteogenesis of human dental pulp derived mesenchymal stem cells. Method The human dental pulp derived mesenchymal stem cells were successfully isolated and were identified in vitro. The TGF-β contained osteogenic inducing system were performed to observe the effect of aspirin in regulating the osteogenesis of human dental pulp derived mesenchymal stem cells; realtime PCR were performed to detect the expression of osteogenesis related transcriptional factors (Runx2, Osterox and ALP) and cell proliferation associated genes (Sox2, Nanog and Oct4). Result By inducing the human dental pulp derived mesenchymal stem cells in osteogenic inducing system for 20 days, the results showed that aspirin can significantly promote human dental pulp mesenchymal stem cells into osteoblasts. With the increasing concentration of aspirin, alkaline phosphatase staining, alkaline phosphatase staining was up-regulated significantly; cloning experiments showed that cell clones decreased significantly; at the same time, real-time PCR showed that the expression of osteogenesis related transcriptional factors (Runx2, Osterox and ALP) was up-regulated significantly, but the expression of the stem cell related genes (Sox2, Nanog and Oct4) was down regulated significantly. Conclusion Aspirin can affect the osteogenic differentiation of human dental pulp mesenchymal stem cells. It can improve the dyeing rate of alkaline phosphatase significantly and up-regulation the expression of osteogenic related transcription factors. In order to promote human dental pulp mesenchymal stem cells into osteoblasts as a breakthrough, it can provide new ideas for the regeneration of dentin.关键词
阿司匹林/人牙髓间充质干细胞/成骨分化Key words
Aspirin/Human dental pulp derived mesenchymal stem cells/Osteogenesis引用本文复制引用
申晓靖,刘海蓉,刘新燕,赵鹏,郑建金,卢恕来,王超..阿司匹林影响人牙髓间充质干细胞成骨分化的新型药理研究[J].中国医学前沿杂志（电子版）,2017,9(1):73-77,5.基金项目
青岛市卫计委资助课题(2014-WJZD022) （2014-WJZD022）
