组织工程与重建外科杂志2017，Vol.13Issue(1)：1-4,4.DOI:10.3969/j.issn.1673-0364.2017.01.001
运用GT/PCL纳米纤维电纺膜在猪皮下构建组织工程化软骨的实验研究
The Construction of Tissue Engineered Cartilage by Using GT/PCL Nanofibres Membranes in Porcine Model
摘要
Abstract
Objective To investigate the possibility of the tissue engineered cartilage construction with electrospun gelatin/polycaprolactone (GT/PCL) membranes in subcutaneous of porcine model. Methods Engineered cartilage was constructed by auricular chondrocytes of porcine as seed cells with GT/PCL membranes (experimental group) and PGA/PLA material (control group) as scaffold. Then the engineered cartilage was implanted subcutaneously in the abdominal wall of swine after cultured 3 weeks in vitro. The samples of the two groups were collected at two time points:3 weeks in vitro and 3 weeks in vivo. Histological observation, wet weight and mechanical strength test were used to evaluate the chondrogenesis. Results After 3 weeks cultured in vitro, cartilage phenotype and undegraded scaffolds were observed in two groups by histological analysis. And the extracellular matrix in control group seems more uniform than the experimental group. However, After 3 weeks cultured in vivo, mature cartilage were only formed in the experimental group, and fibrous tissue substitute for cartilage was observed in the control group. In addition, the engineered cartilage in experimental group showed better mechanical strength. Conclusion GT/PCL membranes could be successfully used in the construction of tissue engineering cartilage in subcutaneous of large animal. It could be a kind of promising scaffold for cartilage tissue engineering.关键词
组织工程化软骨/纳米纤维电纺膜/聚乙酸/聚乳酸/炎症反应Key words
Tissue engineered cartilage/Electrospun nanofibres membranes/Polyglycolic acid/Polylactic acid/Inflammatory response分类
医药卫生引用本文复制引用
霍莹莹,周广东,郑蕊..运用GT/PCL纳米纤维电纺膜在猪皮下构建组织工程化软骨的实验研究[J].组织工程与重建外科杂志,2017,13(1):1-4,4.基金项目
国家自然科学基金(81401532). （81401532）
