组织工程与重建外科杂志2017，Vol.13Issue(1)：5-8,12,5.DOI:10.3969/j.issn.1673-0364.2017.01.002
辣椒素抑制体外培养瘢痕疙瘩成纤维细胞增殖的实验研究
Inhibitory Effect of Capsaicin on the Proliferation of Keloid Fibroblasts Cultured in Vitro
林苗苗 1王文波 2康文 3高振 2武晓莉 2唐胜建 2刘伟1
作者信息
- 1. 261042 山东省潍坊市 潍坊医学院整形外科研究所
- 2. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 3. 上海组织工程研究重点实验室
- 折叠
摘要
Abstract
Objective To explore the effect of capsaicin on inhibiting collagen expression, proliferation and migration ability of keloid fibroblasts in vitro and thus to provide scientific basis for clinical application of capsaicin in treatment of keloid. Methods The keloid tissues were harvested from 9 patients and digested with 0.3% collagenase to extract keloid fibroblasts. These cells were treated with different concentrations of capsaicin (0.5 μg/L and 5 μg/L) as the experiment group or without capsaicin treatment as the control group. CCK-8 assay was used to evaluate cell proliferation. Flow cytometry was also employed to evaluate cell cycle. Q-PCR assay was used to examine the gene expression levels of collagen and other extracellular matrices. In vitro wound scratch was also used to investigate cell migration ability. Results Capsaicin inhibited the proliferation of keloid fibroblasts, resulting in relative blockade of cell cycle in G0/G1 phase. It also inhibited the gene expression of collagens Ⅰ and Ⅲ and fibronectin. In addition, Capsaicin also inhibited the gene expression of IL-6, IL-8 and TNF-α, but had no effect on cell migration. Conclusion Capsaicin could inhibit the proliferation and gene expression of collagen and inflammatory cytokines of cultured fibroblasts in vitro.关键词
瘢痕疙瘩/成纤维细胞/辣椒素/增殖Key words
Keloid/Fibroblasts/Capsaicin/Proliferation分类
医药卫生引用本文复制引用
林苗苗,王文波,康文,高振,武晓莉,唐胜建,刘伟..辣椒素抑制体外培养瘢痕疙瘩成纤维细胞增殖的实验研究[J].组织工程与重建外科杂志,2017,13(1):5-8,12,5.
