组织工程与重建外科杂志2017，Vol.13Issue(1)：13-16,39,5.DOI:10.3969/j.issn.1673-0364.2017.01.004
ADAM10对小鼠颅颌面膜内成骨的影响
The Effects of ADAM10 in Craniofacial Intramembranous Ossification of Mice
摘要
Abstract
Objective To explore the effects of ADAM10 in craniofacial intramembranous ossification of mice. Methods The expression pattern of ADAM10 in mice mandibular was detected by immunofluorescence assay and western blot. Cre-loxp technique was used to detect the function of ADAM10 in intramembranous ossification areas during craniomaxillofacial development. ADAM10 conditional knock-out mice was evaluated by X-ray and von Kossa staining. The cell proliferation, apoptosis and osteoblast differentiation were detected by immunofluorescence assay. Results Immunofluorescence assay revealed that ADAM10 was primarily distributed in the maxillary and mandibular skeletal genesis zones. ADAM10 was abundantly expressed from E14.5 to postnatal day 7 (P7) and was significantly decreased during adulthood. On E15.5, the ADAM10 cKO mice exhibited a shorter snout, flatter posterior skull and smaller maxilla and mandible, compared with the control littermates. The X-ray examination and von Kossa staining shown that the ADAM10 cKO embryos exhibited significantly reduced mineral bone deposition in the maxilla and mandible. Immunofluorescence shown that cell proliferation, apoptosis and osteoblast differentiation were impaired in ADAM10 cKO mice. Conclusion ADAM10, widely expressed in osteogenesis area of mice, can affect the intramembranous bone formation by regulating cell proliferation, differentiation and apoptosis.关键词
解聚素样金属蛋白酶10/颅颌面骨骼/膜内成骨Key words
ADAM10/Maxillofacial bone/Intramembranous bone formation分类
医药卫生引用本文复制引用
谭宇,罗薇,曹海峰,房兵,杨秩..ADAM10对小鼠颅颌面膜内成骨的影响[J].组织工程与重建外科杂志,2017,13(1):13-16,39,5.基金项目
上海市科委面上项目 (14ZR1424100) （14ZR1424100）
国家自然科学基金青年项目 (81000451) （81000451）
上海市卫生局青年科研项目(2010y142) （2010y142）
上海市教委优秀青年教师项目 （）
上海市第九人民医院优青项目 （）
上海市卫计委青年项目(20154Y0181) （20154Y0181）
上海市口腔病防治院面上项目(SSDC-2013-01). （SSDC-2013-01）
