组织工程与重建外科杂志2017，Vol.13Issue(1)：17-20,4.DOI:10.3969/j.issn.1673-0364.2017.01.005
丹参对3T3-L1细胞成脂分化与增殖的影响
Effect of Salvia Miltiorrhiza on Proliferation and Differentiation of 3T3-L1 Cells
林怀安 1余力 1王健 1张波 1郑丹宁 1周佳1
作者信息
- 1. 200011 上海市 上海交通大学医学院附属第九人民医院整复外科
- 折叠
摘要
Abstract
Objective To explore the effect of Salvia miltiorrhiza on proliferation and differentiation of 3T3-L1 cells, so as to provide evidence for further research and clinical application of fat transplantation. Methods The differentiating 3T3-L1 cells after adipogenic induction were incubated with different concentration of S.miltiorrhiza (0.6 g/L, 0.3 g/L, 0.15 g/L, 0.075 g/L, 0.04 g/L, 0.02 g/L). After the 3T3-L1 cells differentiated into mature adipocyte, the cell proliferation viability was determined by CCK-8 assay kit. The lipid droplets accumulation of adipocyte were observed by Oil Red O staining and Image Pro Plus 6.0. The adipogenesis was quantified by measuring lipid content using triglyceride GPO-POD assay kit. Results S. miltiorrhiza promoted a dose-and time-dependent increase in 3T3-L1 cell proliferation viability. The earlier the dosing time was, the better the cell proliferation viability would be; The higher the concentration was, the better the cell proliferation viability would be. Within the six S.miltiorrhiza concentrations confine, S.miltiorrhiza also promoted a dose-dependent increase in 3T3-L1 cell differentiation ability, enhanced the lipid droplets accumulation of 3T3-L1 adipocyte. Conclusion S.miltiorrhiza can promote the increase of adipocyte number via the proliferation and differentiation of 3T3-L1 cells.关键词
丹参/3T3-L1/细胞增殖/细胞分化Key words
Salvia miltiorrhiza/3T3-L1/Cell proliferation/Cell differentiation分类
医药卫生引用本文复制引用
林怀安,余力,王健,张波,郑丹宁,周佳..丹参对3T3-L1细胞成脂分化与增殖的影响[J].组织工程与重建外科杂志,2017,13(1):17-20,4.
