癌变·畸变·突变2017，Vol.29Issue(2)：91-95,5.DOI:10.3969/j.issn.1004-616x.2017.02.003
原花青素对淀粉样蛋白Aβ 25-35诱导的 SH-SY5Y细胞 毒性的影响
The influences of proanthocyanidin on amyloid beta fragment Aβ25-35 induced cytotoxicity in SH-SY5Y cells
摘要
Abstract
OBJECTIVE:and soluble amyloid precursor protein sAPPα which were induced by amyloid protein fragment Aβ25-35 in SH-SY5Y cells was investigated.METHODS:SH-SY5Y cells were treated with Aβ25-35 of different concentrations (0.1,1.0,10.0 and 20.0μmol/L) for 24 hours. Cell viability was measured by MTT assay. Cell injury models were built with 1.0μmol/L Aβ25-35,with proanthocyanidin of different concentrations added to cell cultures and incubated for 24h. Concentrations of Aβ1-42 and sAPPαin cell supernatant were determined by using ELISA. Intracellular content MDA and total SOD were determined by the method of TBA test and WST-8,respectively.RESULTS:Compared with the control group,cells treated with Aβ25-35 (1μmol/L) hadsignificantly declined cell viability (P<0.01),increased Aβ1-42 level (P<0.01),declined sAPPα concentration (P<0.01), increased intracellular MDA content (P<0.01),and decreased total SOD vitality (P<0.01). Compared with the Aβ25-35 injury cell models,proanthocyanidin (0.1,0.5,1.0 and 5.0μg/mL) significantly improved cell viability (P<0.05),decreased the secretion of Aβ1-42 (P<0.01). Proanthocyanidin (0.5,1.0 and 5.0μg/mL) increased sAPPα concentration (P<0.05),decreased MDA content (P<0.05),and strengthened total SOD vitality (P<0.05). CONCLUSION:Proanthocyanidin alleviated the Aβ burden a nd o xidative s tress i n S H-SY5Y c ells w hich w ere i nduced b y Aβ25-35. The influence of proanthocyanidins on oxidative stress,secretion of amyloid protein Aβ1-42.关键词
原花青素/细胞毒性Aβ1-42/sAPPαKey words
proanthocyanidin/Aβ1-42/sAPPα/cytotoxicity分类
医药卫生引用本文复制引用
周亚盼,张小强,曲志华,张妍,梁晓瑜..原花青素对淀粉样蛋白Aβ 25-35诱导的 SH-SY5Y细胞 毒性的影响[J].癌变·畸变·突变,2017,29(2):91-95,5.基金项目
2016年江苏省普通高校研究生科研创新计划资助项目资助(SJZZ16_0034) （SJZZ16_0034）
江苏省卫生厅预防医学科研基金(Y2013070) （Y2013070）
