癌变·畸变·突变2017，Vol.29Issue(2)：96-101,6.DOI:10.3969/j.issn.1004-616x.2017.02.004
华蟾素注射液对 人食管癌细胞增殖、侵袭和化疗敏感性 的影响及其机制
Effect of cinobufacini on chemotherapy sensitivity and toxicity of human esophageal carcinoma cellsin vitro
摘要
Abstract
OBJECTIVE:To investigate the effects of cinobufacini on proliferation,invasion and chemotherapy sensitivity of human esophageal carcinoma,and to analyze its impact on epithelial-mesenchymal transition and its mechanism.METHODS:The effects of cinobufacini on proliferation and chemotherapy sensitivity of human esophagus cancer ECA109 cells were measured by the methyl thiazolyl tetrazolium (MTT) assay. After treatment with cinobufacini, cell apoptosis was analyzed and chemotherapy sensitivity by flow cytometry (FCW). In addition,transwell assay was employed to examine viability,migration and invasion. The effects of cinobufacini on invasion,epithelial-mesenchymal transition of ECA109 and multidrug resistance (MDR)-related proteins were examined by Western blot assay. RESULTS:Cinobufacini treatment significantly inhibited ECA109 cell proliferation in both time- and dose-dependent ways (r=0.985,P=0.000;r=0.988,P=0.000),and enhanced chemotherapy sensitivitycompared to that in the control group (P=0.000). Cell migration and invasion were significantly suppressed (bothP=0.000). In addition,the treatment also down-regulated the expression levels of Snail,Twist,MMP2,Vimentin and P-gp,but up-regulated the expression of E-cadherin.CONCLUSION:Cinobufacini effectively inhibited the proliferation and invasion of human esophageal carcinoma ECA109 cell,and enhance its chemotherapy sensitivity in culture. The mechanism might be partly related to the inhibition epithelial-mesenchymal transition by cinobufacini.关键词
华蟾素/化疗敏感性/上皮-间质转化/增殖/侵袭Key words
cinobufacini/chemotherapy s ensitivity/epithelial-mesenchymal t ransition/proliferation/invasion分类
医药卫生引用本文复制引用
杨兴肖,马鸣,单保恩,李幼梅,宋姮,刘志坤,祝淑钗..华蟾素注射液对 人食管癌细胞增殖、侵袭和化疗敏感性 的影响及其机制[J].癌变·畸变·突变,2017,29(2):96-101,6.基金项目
国家自然科学基金项目(81372416) （81372416）
河北省中医药管理局项目 (2015131) （2015131）
河北省医学科学研究所项目(20160183,20160171) （20160183,20160171）
