癌变·畸变·突变2017，Vol.29Issue(2)：125-128,133,5.DOI:10.3969/j.issn.1004-616x.2017.02.010
红花水提物不同有效部位的体外 抗辐射作用及 机制研究
Radioprotection effects from different extracts of Carthamus tinctorius
摘要
Abstract
OBJECTIVE:To develop new,safe and effective radioprotectors,the protective effects from different water extracts ofCarthamustinctorius were investigated.METHODS:Safflower water extract was prepared with distilled water at 60 ℃ and isolated by HP-20 macroporous resin. Water elution fraction,30%,50%,70% and 95% ethanol elution fractions were separated for the study. The free radical scavenging ability of the different extraction fractions were evaluated by electron spin resonance (ESR) spectra. CCK-8 assay was performed to evaluate the survival rate of human liver L02 cells in vitro. Flow cytometry was applied to study apoptosis,cell cycle and the ratio of Bcl-2/Bax of 60human lymphocyte AHH-1 after Co ay irradiation.RESULTS:The ESR results showed that all the different effective parts of safflower water extract had the ability to scavenge free radicals,with IC50 at 1.7-79.7 mg/mL. The survival rates from the 30% and 50% ethanol elution were 128.3% and 130.9% by the CCK-8 assay. The survival rates were significantly higher than the irradiated group and suggested that the two parts had equal radioprotection effect to the water extract of safflower. The decreased apoptosis rate of AHH-1 cells: the 30% fraction (15.3%±1.6%) and the 50% fraction(15.2%±1.9%) were significantly lower than that of the irradiation group (24.7%±4.4%) (P<0.01). The results of AHH-1 cell cycle analysis showed that the 30% and 50% ethanol elution fractions had significant improvements in the G2/M phase block that was induced by irradiation (P<0.01). At the same time,the two fractions increased the Bcl-2/Bax ratio (P<0.05) and inhibited the cell apoptosis.CONCLUSION:Our data show that the 30% and 50% ethanol elution 60fractions had good radioprotective effects for L02 and AHH-1 cells which were irradiated with Coγrays. The mechanism for the protection may be related to the elimination of free radicals,the improvement of G2/M cell cycle block,and the regulation Bcl-2/Bax ratio in order to inhibit cell apoptosis. The two fractions should be further studied as potentially safe and effective radioprotectors.关键词
红花/有效部位/电子自旋共振波谱仪/CCK-8/作用机制Key words
CarthamustinctoriusL./effective f raction/electron s pin r esonance s pectra/CCK-8/mechanism of radioprotection分类
医药卫生引用本文复制引用
范莉,刘建香,封江彬,姜晓燕,高刚,田梅..红花水提物不同有效部位的体外 抗辐射作用及 机制研究[J].癌变·畸变·突变,2017,29(2):125-128,133,5.基金项目
北京市自然科学基金项目(7142116) （7142116）
