癌变·畸变·突变2017，Vol.29Issue(2)：134-138,142,6.DOI:10.3969/j.issn.1004-616x.2017.02.012
铁过载对性成熟期雄性大鼠生育能力的影响
Effect of iron overload on fertility of sexually mature male rats
摘要
Abstract
OBJECTIVE:The aim of the study was to observe the effect of iron overload on fertility of sexually mature male rats.METHODS:According to bodyweights,40 healthy male Wistar rats were randomly divided into four groups:control,iron deficiency,low-dose iron overloadand high-dose iron overload groups. All rats were injected 2+intraperitoneally every other day. The content of the Fe in these four groups were 0.9,0.3,9 and 18 mg,respectively. After 6 weeks,blood samples from these rats were collected to determine serum iron levels. Testes and epididymides were removed and weighed. Sperm numbers were counted. Sperm mobility and teratosperm rates were calculated. Iron concentration,catalase(CAT),totalsuperoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and malonaldehyde (MDA) levels in the testes were determined.RESULTS:Compared with the control group,the serum iron concentration of the iron deficiency group was significantly lower (P<0.01). However,there was no significant difference in the iron concentrations of the testes (P>0.05). The serum and testes iron concentrations in the low- and high-doses iron overload groups were both significantly higher than that in the control group (all P<0.01). There was no significant difference in the sperm count,the sperm motility rate and the teratosperm rate between the iron deficiency group and the control group (all P>0.05). Compared with the control group,the sperm motility rate in the low-dose iron overload group was significantly decreased (P<0.01);the sperm count and the sperm motility rate in the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the teratosperm rate was significantly higher than that of control group and low-dose iron overload group (allP<0.05). The rats in the iron deficiency group had significantly lower GSH-Px l evels in the testis comparing with the control group. Moreover,compared with the control group,the GSH-Px levels in the testis of the low-dose iron overload group were significantly lower,but the MDA level was significantly higher (all P<0.05). The CAT and GSH-Px levels in the testis of the high-dose iron overload group were all significantly lower than those of control group and low-dose iron overload group,but the MDA level was significantly higher than that of control group and low-dose iron overload group (all P<0.05).CONCLUSION:Iron overload can affect the fertility of sexually mature male rats,including reduced sperm count,decreased sperm motility rate and increased teratosperm rate. These adverse effects may be related to the increased oxidatives tress level caused b y on overload.关键词
铁过载/精子数量/精子活动率/精子畸形率/氧化应激Key words
iron overload/sperm count/sperm motility rate/teratosperm rate/oxidative stress分类
医药卫生引用本文复制引用
张华琦,马爱国,孙永叶,钟凤,蔡静,何丽敏..铁过载对性成熟期雄性大鼠生育能力的影响[J].癌变·畸变·突变,2017,29(2):134-138,142,6.基金项目
中国博士后科学基金面上资助项目(2015M582059) （2015M582059）
青岛市博士后应用研究项目(2015171) （2015171）
青岛大学医学院创新团队青年教师培育项目 （）
