安徽农业大学学报2017，Vol.44Issue(1)：1-6,6.DOI:10.13610/j.cnki.1672-352x.20170208.003
短期遮阴对茶树嘌呤碱、氨基酸和儿茶素生物合成的影响
Short-term shading influencing the biosynthesis of caffeine, theanine and catechins in tea (Camellia sinensis)
摘要
Abstract
In this paper,we investigated the influence of short-term shading on the biosynthesis of purine alkaloids,amino acids and catechins.We analyzed the content of three major metabolites (the metabolisms of purine alkaloids,amino acids and polyphenols) and the expression of key genes in biosynthesis pathways in tea (Camellia sinensis) by HPLC (high performance liquid chromatography) and fluorescence quantitative PCR.Our data revealed that short-term shading slightly down-regulated the total catechins and restrained the biosynthesis of caffeine.Moreover,short-term shading had notable effects on amino acids biosynthesis by increasing 58.5％.The content of theanine had about sixfold increase compared with the control group,and it was remarkably correlated with the expression of some genes,such as TCS1,PAL,F3'H and ANR2 according to the differential expression of these genes.The results showed that shading has certain relation with the biosynthesis of purine alkaloids,amino acids and polyphenols in rooted tea cuttings.关键词
高效液相色谱/荧光定量PCR/咖啡碱/氨基酸/多酚类化合物/生物合成Key words
HPLC/qRT-PCR/caffeine/amino acid/polyphenols/biosynthesis分类
农业科技引用本文复制引用
顾辰辰,王荣秀,江丽娜,邓威威..短期遮阴对茶树嘌呤碱、氨基酸和儿茶素生物合成的影响[J].安徽农业大学学报,2017,44(1):1-6,6.基金项目
国家自然科学基金(31300576)资助. （31300576）
