安徽农业大学学报2017，Vol.44Issue(1)：22-26,5.DOI:10.13610/j.cnki.1672-352x.20170208.024
纤维素酶Umcel9y-1在银杏叶总黄酮提取中的增效作用
Synergized action of cellulase Umcel9y-1 on the extract efficiency of total flavonoid from Ginkgo biloba L.leaves
摘要
Abstract
Ginkgo leaves were selected as experimental materials to investigate the cellulase Umcel9y-1 synergized action on total flavonoids extraction.Under the optimum reaction conditions (pH 7.0,37 ℃),the extraction yields of total flavonoids increased by 20.3％,20.7 ％,16.8％ and 15％ of the corresponding control after the leaves were treated with 0.08 mg·mL-1 Umcel9y-1 for 1 h,2 h,4 h and overnight (12 h).The extraction yield oftoal flavonoids in the 2-hour treatment group reached 97.01％ of the overnight-treatment of the control.The results showed the extract efficiency was greatly improved by the addition of cellulase Umcel9y-1.Moreover,the medical ingredients of the extracts were evaluated by Ultra performance liquid chromatography (UPLC),indicating that Umcel9y-1 had no obvious influence on the medical ingredients of the extract compared to the corresponding control.关键词
纤维素酶/银杏叶/总黄酮/提取Key words
cellulase/Ginkgo leaf/total flavonoid/extraction分类
医药卫生引用本文复制引用
王旭,王志,蔡梦宇,邬艳博,周育..纤维素酶Umcel9y-1在银杏叶总黄酮提取中的增效作用[J].安徽农业大学学报,2017,44(1):22-26,5.基金项目
安徽省自然科学基金(1608085QC57)和安徽省杰出青年基金(1608085J08)共同资助. （1608085QC57）
