安徽农业大学学报2017，Vol.44Issue(1)：44-49,6.DOI:10.13610/j.cnki.1672-352x.20170208.027
骨髓间充质干细胞移植在卵巢早衰小鼠卵巢功能重建中应用
Transplatation of bone marrow mesenchymal stem cells for reconstruction of the ovarian function in the premature ovarian insufficiency mice
摘要
Abstract
The purpose of this study was to evaluate the application value of bone marrow mesenchymal stem cells (BMSCs) transplantation to ovarian function reconstruction in the premature ovarian insufficiency/failure (POI/POF) mice.The model of mouse POF was established based on a single intraperitoneal injection of cyclophosphamide (120 mg·kg-1) and busulfan (12 mg·kg1).Ninety mice were randomly divided into three groups:POF model group,BMSCs transplantation group and blank control group.On the 14th day of post-administrated chemotherapy drugs,mice in the BMSCs transplantation group were injected with 5×107 cell·mL-1 derived from green fluorescent protein (GFP) transgenic mouse via vena caudalis once every four days for five times;meanwhile an equal volume of physiological saline was injected to the mice in the POF model group and the blank control group.Physiological characteristics,serum concentration of FSH and E2,ovarian histology and distribution of green fluorescence signal in the ovarian tissues were assessed in all three groups.The results showed that following anorexia,fatigue and lethargy,the average weight of mice in the POF model group decreased significantly on the 14th day of post-administrated chemotherapy drugs as compared to the blank control group (P＜0.05).Compared with the blank control group,the concentration of FSH increased significantly (P＜0.05),concentration of E2,the number of primordial,growing and mature follicles reduced significantly (P＜0.05),and ovarian interstitial fibrosis was observed in the POF model group.After BMSCs transplantation,the average weight of mice in the BMSCs transplantation group increased gradually with the improvement of feeding,sleeping and activities.Twenty-eight days after the first injection of BMSCs,the serum concentration of FSH was lower than that in the POF model group (P＜0.05),while the concentration of E2,amounts of primordial,growing as well as mature follicles in the BMSCs transplantation group were significantly higher than those in the POF model group (P＜0.05).Granular green fluorescence signal was found dispersedly around follicles under the fluorescence microscope.It was suggested that the transplanted BMSCs could localize and survive in the damaged ovary,and promote the reconstruction of ovarian histological structure and endocrine function.关键词
骨髓间充质干细胞/干细胞移植/卵巢早衰Key words
bone marrow mesenchymal stem cell/stem cell transplantation/premature ovarian insufficiency/failure分类
医药卫生引用本文复制引用
张莹莹,殷慧群,倪丰,姜宏..骨髓间充质干细胞移植在卵巢早衰小鼠卵巢功能重建中应用[J].安徽农业大学学报,2017,44(1):44-49,6.基金项目
南京军区医学科技创新课题(11Z010)资助. （11Z010）
