安徽农业大学学报2017，Vol.44Issue(1)：119-123,5.DOI:10.13610/j.cnki.1672-352x.20170208.023
实时荧光定量PCR检测转基因玉米Bt176测量不确定度分析
Uncertainty in detecting event-specific fragments of genetically modified maize BT176 by quantitative real time PCR
摘要
Abstract
The real-time fluorescent quantitative PCR method was used to detect the event-specific fragments in a sample of genetically modified maize BT176 and the uncertainty of measurement derived from several aspects was analyzed.The results showed that uncertainty of type A and type B was 0.0003 and 0.0007,respectively;the combined standard uncertainty was 0.001;the effective degree of freedom was 272;the coverage probability was 95％;the expanded uncertainty was 0.001,and the measured result was 0.8％±0.2％.This result indicated that the main source of uncertainty was the trace amount of liquid transfer,which should be paid more attention to in future detection.关键词
转基因玉米Bt176/实时荧光定量PCR/定量检测/不确定度评估Key words
genetically modified maize Bt176/real-time fluorescent quantitative PCR method/quantitative detection/uncertainty evaluation分类
农业科技引用本文复制引用
熊娟,黄启红,伍玲燕,蔡大川,张志军,黄璇莹,陈敏儿..实时荧光定量PCR检测转基因玉米Bt176测量不确定度分析[J].安徽农业大学学报,2017,44(1):119-123,5.基金项目
广东省省级科技计划项目(2014B070705001)和广东专业镇中小微企业食品安全与质量控制检测服务平台(2013B091604003)共同资助. （2014B070705001）
