安徽农业大学学报2017，Vol.44Issue(1)：135-141,7.DOI:10.13610/j.cnki.1672-352x.20170208.011
墨兰FT同源基因的时空表达及功能分析
Temporal and spatial expression and functional analysis of FT homologous genes in Cymbidium sinense
摘要
Abstract
The FLOWERING LOCUS T (FT) gene plays a central role in integrating flowering signals in plants because its expression is regulated antagonistically by the photoperiod,vernalization pathways and autonomous pathway.We studied the temporal and spatial expression pattems and biological function of homologous gene FT which was isolated from Cymbidium Sinense ‘Qijian Heimo’.It showed that the gene contained 618 bp nucleotides with a 531 bp open reading frame (ORF),encoding 176 amino acids (AAs).The CsFT gene was expressed in all organs of C Sinense ‘Qijian Heimo’,and the CsFT transcript level was higher in axillary buds than in other organs at vegetative stage.At reproductive stage,the expression of CsFT was the highest in axillary bud,scape,flower bud,while the lowest in root and leaf.Over-expression of CsFT in Arabidopsis thaliana showed that the CsFT transgenic lines bloomed earlier than that of the WT plants.关键词
墨兰/FT基因/基因表达Key words
Cymbidium sinense/FT gene/gene expression分类
农业科技引用本文复制引用
黄玮婷,吴博文,方中明..墨兰FT同源基因的时空表达及功能分析[J].安徽农业大学学报,2017,44(1):135-141,7.基金项目
国家自然科学基金(31301250)和湖北省教育厅科学技术研究项目(B2015392,B2015394)共同资助. （31301250）
