安徽农业科学Issue(2)：162-165,4.
飞机草总黄酮提取工艺及其急性毒性研究
Extraction Trocess Optimization and Acute Toxicity of Total Flavones from Eupatorium odoratum Linn
摘要
Abstract
[Objective] To optimize the extraction process of total flavones from Eupatorium odoratum Linn and study the acute toxicity of the total flavones extract to mice.[Method] The extraction yield of the total flavones was taken as the key index,using the ultrasonic assisted ethanol solvent extraction method,the extraction time,extraction temperature,liquid to solid ratio and ethanol concentration were optimized through single factor test and orthogonal test design,the optimum process of the total flavones extract from Eupatorium odoratum Linn was selected.And a range of KM mice were orally administered with the total flavones extract from Eupatorium odoratum Linn to determine the median lethal dose (LD50) and maximum tolerated dose.[Result] The optimized extraction process for the total flavones fromwere the extraction time of 5 h,extraction temperature of 70 ℃,liquid to solid ratio of 1 ∶ 60 and solvent of 70％ ethanol,and the total flavones extraction rate was 7.13％.The oral acute toxici ty of the total flavones extracted from Eupatorium odoratum Linn to KM mice was not determined and the maximum tolerated dose was 80 000 mg/ml.[Conclusion] The total flavones from Eupatorium odoratum Limn was the actual non-toxic substances.关键词
飞机草/总黄酮/提取工艺/急性毒性/小鼠Key words
Eupatorium odoratum Linn/Total flavones/Extraction process/Acute toxicity/Mice分类
农业科技引用本文复制引用
王小宁,陈进军,叶绍棠,郑锦庆,巩栋梁,俞云鹏,林红英..飞机草总黄酮提取工艺及其急性毒性研究[J].安徽农业科学,2016,(2):162-165,4.基金项目
湛江市财政资金科技专项竞争性分配项目(A14031). （A14031）
