安徽农业科学2017，Vol.45Issue(5)：1-3,3.
丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究
Study on Complex Mutagenesis of Clostridium acetobutylicum and Fermentation of Butanol from Waste Materials
摘要
Abstract
[Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources.[Method] Clostridium acetobutylicum UM-80,a mutant with high butanol yield and good stability,was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe2+ by Clostridium acetobutylicum CGMCC 1.0134.Then the different fermentation materials were used to study the fermentation performance of the mutant strains,and the suitable medium with high cost performance was found out.[Result] The yield of butanol and total solvent (acetone,butanol,ethanol) were 9.04 and 17.95 g/L,respectively,which were 5.12％ and 4.12％ higher than those of the original strain.The fern root was the best raw material for the fermentation of butanol;when the mass fraction of fern root was 15％,the concentration of butanol in fermentation broth was 5.50 g/L.When the co-fermentation of Mortierella alpina and fern root,the highest yield was 6.50 g/L.[Conclusion] The co-fermentation of Mortierella alpina and fem root is better fermentation medium for butanol.关键词
丙酮丁醇梭菌/复合诱变/废弃物原料/丁醇Key words
Clostridium acetobutylicum/Compound mutagenesis/Waste raw material/Butanol分类
农业科技引用本文复制引用
毛碧飞,袁丽霞,陈祥松,孙立洁,朱微微,吴金勇,刘伟伟..丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究[J].安徽农业科学,2017,45(5):1-3,3.基金项目
合肥物质科学技术中心方向项目培育基金(2014FXCX006) （2014FXCX006）
安徽省淮南市科技计划项目(2014A15) （2014A15）
中国科学院合肥分院等离子体物理研究所科学基金(DSJJ-15-YY02). （DSJJ-15-YY02）
