安徽农业科学2017，Vol.45Issue(5)：54-56,3.
苦瓜子衣色素蛋白提取及抗氧化能力研究
Study on Antioxidant Effects and Extracting of Momordica charantia Sub-skin Pigment Protein
摘要
Abstract
[Objective] To study the best condition for the extraction and antioxidant activity of sub-skin pigment from Mordica charantia,to provide a reference for the development and application of natural pigment.[Method] Extracted Momordica charantia sub-skin pigment with ethyl acetate,extracted the protein with aqueous two-phase system,purified it by DEAE-52 anion chromatography and Sephadex G-100 gel column chromatography,determined the antioxidant effects by Fenton method.[Result] The best condition revealed that:temperature 50 ℃,proportion of solid to liquid is 1∶40 (g∶ mL),time 30 min,the extraction rate up to 45.54％.The condition of aqueous two-phase system was:35％ ammonium sulfate and polyethylene glycol of 30％,purified the protein with molecular weight 74.10 kD.The antioxidant tests indicated that Momordica charantia sub-skin pigment protein had antioxidant effects and their eliminate capacity increased with the concentration of solution increased.[Conclusion] This test used a new aqueous two-phase system to extract the protein,simplified the extraction process and reduced the loss of protein.The antioxidant test provided a theoretical basis for the preparation of high quality and non polluting antioxidant preparation from natural pigments.关键词
苦瓜子衣色素蛋白/分离纯化/柱层析/抗氧化作用Key words
Momordica charantia sub-skin pigment protein/Separation and purification/Column chromatography/Antioxidant分类
农业科技引用本文复制引用
于群,张惠,安磊,杨浩宇..苦瓜子衣色素蛋白提取及抗氧化能力研究[J].安徽农业科学,2017,45(5):54-56,3.基金项目
宁夏职业技术学院(宁夏广播电视大学)科研发展基金项目. （宁夏广播电视大学）
