安徽医科大学学报2017，Vol.52Issue(1)：7-12,6.DOI:10.19405/j.cnki.issn1000-1492.2017.01.002
全反式维甲酸对实验性动脉粥样硬化兔动脉血管内皮依赖性舒张功能的影响
Effect of all-trans-retinoic acid on endothelial-dependent dilation in atherosclerosis rabbits
摘要
Abstract
Objective To investigate the effects of all-trans-retinoic acid ( ATRA ) on endothelial-dependent dila-tion in experimental atherosclerosis rabbits. Methods New Zealand white rabbits were randomly divided into 3 groups:the normal group were fed on standard diet;high-fat diet model group were fed on high-fat diet ( standard diet supplemented with 1℅ cholesterol+5℅ lard);ATRA group were fed on high-fat diet and 5 mg/( kg·day) administered orally. After 2, 4, 8, 12 weeks, experimental rabbits were sacrificed to get blood and blood lipids were tested with a kit;the formation of lipid plaque was observed by oil red staining and the change of endothelial structure was watched by HE staining. After 8 weeks, 3 rabbits of each group were randomly assigned to detect en-dothelial-dependent dilation and non-endothelial-dependent dilation on isolated arterial rings. Results Compared with the normal group, after 2 weeks, the concentration of total cholesterol( TCH) and LDL-C in model group in-creased significantly (P<0. 05). The concentration of triglyceride(TG) and HDL-C increased significantly after 4 weeks (P<0. 05);Oil red staining showed that in two weeks, lipid plaque was not obvious;4 weeks later, the lipid plaque was increased;HE staining showed that the intima was thickened with the passage of time; the foam cells increased;the vascular smooth muscle cell polarity was disordered, and migrating to the intima;the change of EDD indicated that the model group attenuated conspicuously (P<0. 05). Compared with the model group, in AT-RA group, there was no difference in the concentration of TG. The concentration of TCH decreased significantly ( P<0. 05 );the concentration of HDL-C for 4 weeks and 12 weeks significantly increased ( P<0. 05 ) . The level of LDL-C for 2 weeks and 12 weeks reduced ( P<0. 05 ); Oil red staining revealed that the lipid plaque was visible after 4 weeks;HE staining indicated that compared with the model group, the intima of ATRA group was slightly thickened, foam cells were reduced, and arrangement of the smooth muscle cells was more neatly;when the level of Ach was 10 -7 mol/L, the EDD of ATRA group was significantly higher than that in the model group ( P <0. 05). Conclusion Hyperlipidemia is a cause of atherosclerosis. The damaged artery endothelium makes endo-thelial-dependent dilation decreased, and ATRA might improve endothelium dependent relaxing function by reduc-ing serum lipids.关键词
全反式维甲酸/动脉粥样硬化/内皮依赖性舒张功能Key words
ATRA/atherosclerosis/endothelial-dependent dilation分类
医药卫生引用本文复制引用
丁延辉,丁俊丽,周青,汪渊..全反式维甲酸对实验性动脉粥样硬化兔动脉血管内皮依赖性舒张功能的影响[J].安徽医科大学学报,2017,52(1):7-12,6.基金项目
国家自然科学基金(编号:81470568) （编号:81470568）
