安徽医科大学学报2017，Vol.52Issue(1)：13-17,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.003
复方血竭制剂对小鼠溃疡性结肠炎相关结直肠癌的疗效及可能机制
Efficacy and mechanism of compound dracorhodin on ulcerative colitis-related colorectal cancer in mice
摘要
Abstract
Objective To investigate the therapeutic effects and mechanism of compound dracorhodin on experi-mental ulcerative colitis related colorectal cancer( UCRCC) . Methods Sixty male BALB/c mice were randomly as-signed to four groups(n=15):blank control group, model group, low dose of compound dracorhodin group and high dose of compound dracorhodin group. Disease activity index( DAI) was evaluated daily during the experiment. Mice were sacrificed after 11 weeks,from which the colons and rectums were removed. The colon histomorphology was observed by naked eyes and microscope. The expressions of β-catenin, c-myc and PCNA were detected by Western blot and real time quantitative PCR. Results In the model group, obvious congestion, edema and polyps were found by naked eyes;lots of inflammatory cells, different degrees of intraepithelial neoplasia and even carci-noma were observed under microscope. In the compound dracorhodin groups, mucosal congestion, edema were ob-served, and the number of polyps was reduced;inflammatory cells and a small amount of low-grade intraepithelial neoplasia were found under the microscope, no cancer was observed. Compared with the blank group, the DAI scores, nucleus localization ofβ-catenin protein, the mRNA transcription and protein expression level of c-myc and PCNA were significantly higher(P<0. 05) in the model group and the treatment groups(P<0. 05). There was no significant difference between the compound dracorhodin groups, but significantly decreased, compared with the model group ( P <0. 05 ) . The transcription level of β-catenin showed no significant difference among 4 groups. Conclusion Compound dracorhodin reduced malignant transformation of experimental ulcerative colitis at least partially through relieving the inflammatory reaction and inhibiting the Wnt/β-catenin pathway.关键词
复方血竭/溃疡性结肠炎相关结直肠癌/β-cateninKey words
compound dracorhodin/ulcerative colitis related colorectal cancer/β-catenin分类
医药卫生引用本文复制引用
李方,郑楠,赵亚娇,王雪明,翟俊山,朱超慧,李楠..复方血竭制剂对小鼠溃疡性结肠炎相关结直肠癌的疗效及可能机制[J].安徽医科大学学报,2017,52(1):13-17,5.基金项目
北京市自然科学基金(编号:7132175) （编号:7132175）
