安徽医科大学学报2017，Vol.52Issue(1)：37-41,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.008
CSE过表达保护低氧/无血清诱导骨髓间充质干细胞凋亡的机制
Mechanisms of CSE overexpression protecting mesenchymal stem cells from hypoxia and serum deprivation-induced apoptosis
摘要
Abstract
Objective To explore the mechanisms of cystathionine-γ-lyase( CSE) overexpression protecting mesen-chymal stem cells(MSCs) from hypoxia and serum deprivation(H/SD)-induced apoptosis. Methods Rat bone marrow MSCs were isolated and cultured in vitro. MSCs were treated with H/SD as an apoptosis model. Plv-Zs-Green-CSE lentivirus and Plv-ZsGreen lentivus vector were constructed and transfected to MSCs( CSE MSCs and GFP M-SCs) . The cells were divided into Norm MSCs group( normal cultured MSCs) ,CSE MSCs group,GFP MSCs group. Apop-tosis of cells was detected by using flow cytometry analysis, Western blot were utilized to determine the protein lev-els of Cyt c,Bcl-2,Bax after H/SD treatment in MSCs. Results CSE overexpression was mediated by lentiviral transduction in MSCs. Compared with GFP MSCs or Norm MSCs group, CSE protein expression in CSE MSCs group was higher markedly(P<0. 01). Afterward, the modified MSCs were treated under H/SD for 12 h. Compared with GF-PMSCs or NormMSCs group. CSEMSCs group had a significant lower proportion of apoptosis(P<0. 01). Bcl-2 protein expressionin CSEMSCs group was markedly upregulated compared with GFPMSCs or NormMSCs group(P<0. 01). Bax protein expression in CSEMSCs group was significantly downregulated compared with GFPMSCs or NormMSCs group(P<0. 01). Furthermore, we found that CSEMSCs group decreased significantly cytochrome c accumulation in the cy-tosol compared with NormMSCs group or GFPMSCs group(P<0. 01). Above parameters were similar between GFPMSCs and Norm MSCs group. Conclusion CSE overexpression protects mesenchymal stem cells from hypoxia and serum deprivation-induced apoptosis via attenuaion of the mitochondrial apoptpsis pathway.关键词
骨髓间充质干细胞/胱硫醚-γ-裂解酶/凋亡/机制Key words
mesenchymal stem cells/cystathionine gamma-lyase/apoptosis/mechanism分类
医药卫生引用本文复制引用
李从圣,王爱玲,杨静,郝玉瑜,郭增..CSE过表达保护低氧/无血清诱导骨髓间充质干细胞凋亡的机制[J].安徽医科大学学报,2017,52(1):37-41,5.基金项目
安徽高校自然科学研究项目(编号:kj2015Z024) （编号:kj2015Z024）
北京协和医学基金-睿E(睿意)急诊医学研究专项基金(编号:R2015012) （睿意）
安徽医科大学校基金(编号:2015xkj062) （编号:2015xkj062）
