安徽医科大学学报2017，Vol.52Issue(1)：42-46,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.009
光甘草定对结直肠癌细胞RKO增殖和凋亡的影响
Effects of glabridin on the proliferation and apoptosis of colorectal cancer cells
摘要
Abstract
Objective To explore the effects of glabridin on the proliferation of human colorectal cancer cell line RKO and the probable mechanisms. Methods MTT assay was used to detect the proliferation of RKO cells treated with different concentrations of glabridin. Hoechst staining was used to detect the apoptosis of RKO cells. The ex-pression of mitogen-activated protein kinase ( MAPK ) signaling pathway proteins and the proteins associated with apoptosis B-cell lymphoma-2 ( Bcl-2 ) , Bcl-2 assaciated X protein ( Bax ) , cysteinyl aspartate specific proteinases (Caspase-8, Procaspase-9, Caspase-3) in RKO cells were detected by Western blot. Results Glabridin inhibited the proliferation of RKO cells in a dose-and time-dependent manner compared with cell group(P<0. 05). Glabri-din regulated the expression of MAPKs. Glabridin induced the apotosis of RKO cells,down-regulated the expression of Bcl-2 and Procaspase-9 and up-regulated the expression of Bax and Caspase-3 compared with the control group (P<0. 05), while Caspase-8 expression was constant in all groups. Conclusion Glabridin exhibits an inhibitory effect on RKO cells proliferation. Glabridin promotes the apoptosis of RKO cells, which may regulate the expression of the proteins associated with apoptosis through MAPK signaling pathway.关键词
结直肠癌/RKO细胞/光甘草定/凋亡Key words
colorectal cancer/RKO cells/glabridin/apotosis分类
医药卫生引用本文复制引用
杨小平,邹多兵,丁延辉,左莉,周青,汪渊..光甘草定对结直肠癌细胞RKO增殖和凋亡的影响[J].安徽医科大学学报,2017,52(1):42-46,5.基金项目
国家自然科学基金(编号:81272399 ) （编号:81272399 ）
安徽省自然科学基金(编号:1508085QH167) （编号:1508085QH167）
