安徽医科大学学报2017，Vol.52Issue(1)：62-66,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.013
CuSO4对HepG2细胞相关炎症指标的影响
Influences of copper sulfate on related inflammatory markers of HepG2 cells
摘要
Abstract
Objective To observe the influences of copper sulfate on related inflammatory markers such as nuclear factor-κB( NF-κB) of HepG2 cells, the cell lines of hepatocellular carcinoma( HCC) . Methods HepG2 cells were cultured by RPMI-1640 contained various concentrations of copper sulfate in vitro. MTT colorimetric assay was used to detect the growth inhibitory effect of copper sulfate on HepG2 cell. Western blot was used to estimate the effect of copper sulfate on changes of related inflammatory markers, for instance, NF-κBp65, interleukin-1β(IL-1β), interleukin -4 ( IL-4 ) , interleukin-5 ( IL-5 ) , interleukin-6 ( IL-6 ) and γ-interferon ( IFN-γ) . Results The growth of HepG2 cells was inhibited by copper sulfate in certain concentrations. The expression levels of NF-κB, IFN-γand IL-5 were reduced and IL-1β, IL-4 and IL-6 were up-regulated in HepG2 cells by copper sulfate with a dose-dependent manner. Conclusion Copper sulfate may inhibit the proliferation of HepG2 cells. And the inflammatory reaction in HepG2 cells can be suppressed by copper sulfate through lowering the levels of NF-κB, IFN-γ and IL-5 and increasing the levels of IL-1β, IL-4 and IL-6 .关键词
CuSO4/HepG2细胞/肝细胞癌/炎症/核因子-κBKey words
copper sulfate/HepG2 cells/hepatocellular carcinoma/inflammation/nuclear factor-κB分类
医药卫生引用本文复制引用
张亮亮,董健健,徐陈陈,赵静,汪世靖,程楠,王训,韩咏竹..CuSO4对HepG2细胞相关炎症指标的影响[J].安徽医科大学学报,2017,52(1):62-66,5.基金项目
国家自然科学基金(编号:81573954) （编号:81573954）
