安徽医科大学学报2017，Vol.52Issue(1)：83-85,3.DOI:10.19405/j.cnki.issn1000-1492.2017.01.017
miR-210对乳腺癌细胞生物学行为的影响
Effect of miR-210 on biological behavior of breast cancer cell
摘要
Abstract
Objective To explore the effect of microRNA-210 (miR-210) on proliferation, invasion and apoptosis of breast cancer cell. Methods The plasmid expression vector of short hairpin RNA ( shRNA) targeting miR-210 was constructed and transfected into MCF-7 cell by LipofectamineTM2000. The expression of miR-210 was detected by Real-time fluorescence quantitative PCR method after transaction, and proliferation, invasion, apoptosis of MCF-7 cell were observed. Results The expression of miR-210 decreased significantly in MCF-7 cell, and prolif-erative and invasive ability weakened, and cell apoptosis rate increased after transfection with miR-210 shRNA. Conclusion miR-210 expression can affect biological behavior of breast cancer.关键词
microRNA-210/乳腺癌/增殖/侵袭/凋亡Key words
microRNA-210/breast cancer/proliferation/invasion/apoptosis分类
医药卫生引用本文复制引用
蓝永洪,牛海艳,王晗,杨智,江朝娜,符碧薇..miR-210对乳腺癌细胞生物学行为的影响[J].安徽医科大学学报,2017,52(1):83-85,3.基金项目
海南医学院科研培育基金(编号:HY2013-12 ) （编号:HY2013-12 ）
海南省自然科学基金(编号:814301) （编号:814301）
