安徽医科大学学报2017，Vol.52Issue(1)：86-90,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.018
多柔比星对心肌细胞的损伤作用及对microRNA的影响
Effects of doxorubicin on cardiomyocytes and the expression of microRNAs
摘要
Abstract
Objective To investigate the cardiotoxic effects of doxorubicin on neonatal rat cardiomyocytes and its influence on the expression of microRNAs. Methods The neonatal rat cardiomyocytes were cultured for 72 h, then randomly divided into six groups as follow: control group, doxorubicin groups at different concentrations (0. 125, 0. 25,0. 5,1,2 mol/L) . The cells were cultured in normal condition in control group, while the cells in doxorubi-cin groups were pretreated with doxorubicin at different concentrations for 24 h. Beating frequency of cardiomyocytewas observed using an inverted microscope. The cell viability was detected by CCK-8 kit and the lactic dehydrogen-ase( LDH) activity in the culture medium was assessed by microplate. The expressions of miRNAs, including miR-133a-5p, miR-133b-5p, miR-6216 and miR-30e-5p were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results Compared with the control group, doxorubicin significantly decreased the beating frequency of cardiomyocytes and cell viability, while markedly elevated the LDH activity in the Supernatant (P<0. 05). In addition, the expressions of miR-133b-5p, miR-6216 and miR-30e-5p were markedly up-regula-ted , while the expression of miR-133 a-5 p was down-regulated by doxorubicin at the concentration of 1 mol/L signif-icantly. Conclusion Doxorubicin might exert cardiotoxic effects on the neonatal rat cardiomyocytes via regulating the expressions of miR-133a-5p,miR-133b-5p,miR-6216 and miR-30e-5p.关键词
多柔比星/心肌毒性/乳鼠心肌细胞/微小RNAKey words
doxorubicin/cardiotoxicity/neonatal rat cardiomyocytes/microRNA分类
医药卫生引用本文复制引用
杨婉,金世云,许士进,张野,何淑芳..多柔比星对心肌细胞的损伤作用及对microRNA的影响[J].安徽医科大学学报,2017,52(1):86-90,5.基金项目
国家自然科学基金青年科学基金项目(编号:81200171 ) （编号:81200171 ）
安徽高校省级自然科学研究项目(编号:KJ2014ZD16) （编号:KJ2014ZD16）
