安徽医科大学学报2017，Vol.52Issue(1)：95-99,5.DOI:10.19405/j.cnki.issn1000-1492.2017.01.020
siRNA沉默己糖激酶-2基因对MDA-MB231细胞放疗敏感性的影响
Effects of silencing HK2 gene through siRNA on radiosensitivity of MDA-MB231 cell line
摘要
Abstract
Objective To investigate the radio sensitivity of silencing HK2 gene through siRNA interference in human breast cancer MDA-MB231 cells. Methods The cells were divided into three groups: the blank group( Control) ,the negative control group ( Negative) and the test group ( HK2-siRNA) . The HK2 siRNA was transfect-ed into MDA-MB231 cells and the Western blot and qRT-PCR tests were used to detect expressions of HK2 protein and mRNA before and after silencing. Then proliferation of cells in three groups that were exposed to different doses of X-ray (0,2,4,6,8 Gy) was analyzed by CCK-8 experiment,and the apoptosis rate of cells irradiated by 4 Gy X rays was determined by flow cytometry. Results After silencing HK2 gene,the expressions of protein and mRNA of HK2 in the test group cells were inhibited effectively. After three groups of cells being exposed to different doses of X-ray, the survival rate of cells displayed a decreased trend in a dose-dependent manner. And compared with the negative control group,the cell survival rate in the test group was significantly decreased and the cell apoptosis rate in the test group was higher than that in the negative control group and blank group(P<0. 01). Conclusion Si-lencing HK2 gene through siRNA can significantly sensitize MDA-MB231 breast carcinoma cells to X radiation.关键词
己糖激酶-2/siRNA/乳腺癌/放疗敏感性Key words
HK2/siRNA/breast cancer/radiation分类
医药卫生引用本文复制引用
张丹,徐慧琴,汪会,余文静,薛杨央,赵学峰..siRNA沉默己糖激酶-2基因对MDA-MB231细胞放疗敏感性的影响[J].安徽医科大学学报,2017,52(1):95-99,5.基金项目
国家自然科学基金(编号:81371587) （编号:81371587）
