安徽医科大学学报2017，Vol.52Issue(1)：99-104,6.DOI:10.19405/j.cnki.issn1000-1492.2017.01.021
蜕皮甾酮对同型半胱氨酸诱导人血管内皮细胞凋亡的影响
Protective effect of ecdysterone against homocysteine-induced apoptosis of HUVEC
摘要
Abstract
Objective To investigate the effect of ecdysterone ( EDS) against homocysteine( Hcy)-induced apopto-sis of human umbilical vein endothelial cells(CRL-1730). Methods CRL-1730 cells were cultured in vitro routine-ly with different concentrations of EDS. The viability of CRL-1730 cells was detected by cell counting Kit-8 assay. Hcy treatment induced apoptosis in CRL-1730 cells, CRL-1730 cells were divided into 5 groups: control group, high dose of EDS ( Hcy+1. 5 μmol/L EDS ) , middle dose of EDS group ( Hcy+1. 0 μmol/L EDS ) , low dose of EDS group(Hcy+0. 5 μmol/L EDS), and Hcy group. The apoptosis of CRL-1730 cells was determined by flow cytometry assay and was observed by fluorescent staining. Real-time quantitative reverse transcriptase polymerase chain reaction was used to detect Bax, Bcl-2 and Caspase-3 mRNA expressions in CRL-1730 cells. Western blot was used to detect Bax, Bcl-2, cleaved-Caspase-3 protein expressions in CRL-1730 cells. Results EDS in selec-tive concentration had no effect on the viability of CRL-1730 cells. The apoptosis rates of CRL-1730 cells were sig-nificantly increased with the treatment of Hcy, but Hcy-induced apoptosis in CRL-1730 cells was inhibited by EDS treatment in a dose-dependent manner. The protein expressions of Bax and cleaved-Caspase-3 in CRL-1730 cells in the Hcy group showed significant elevation in comparison with the control group. The expression of Bcl-2 protein declined in the Hcy group compared with the control group, but high, middle and low doses of EDS treatments down-regulated the protein expressions of Bax, cleaved Caspase-3 and up-regulated the expression of Bcl-2 in Hcy-treated CRL-1730 cells. The mRNA expressions of Bax and Caspase-3 of CRL-1730 cells in the Hcy group were el-evated significantly in comparison with the control group. The expression of Bcl-2 mRNA was reduced in the Hcy group compared with the control group, but high,middle and low doses of EDS treatments down-regulated the mR-NA expressions of Bax, Caspase-3 and up-regulated the mRNA expression of Bcl-2 in Hcy-treated CRL-1730 cells. Conclusion EDS has a protective effect on Hcy-induced apoptosis of human umbilical vein endothelial cells. This study provides evidence for the efficacy of EDS in the treatment of cardiovascular disease.关键词
蜕皮甾酮/同型半胱氨酸/人脐静脉内皮细胞/凋亡Key words
ecdysterone/homocysteine/human umbilical vein endothelial cells/apoptosis分类
医药卫生引用本文复制引用
苏华,谢睿彬,张小新,罗高湖,凃铃..蜕皮甾酮对同型半胱氨酸诱导人血管内皮细胞凋亡的影响[J].安徽医科大学学报,2017,52(1):99-104,6.基金项目
湖北省卫生计生委员会西医一般项目(编号:WJ2015MB-006) （编号:WJ2015MB-006）
