安徽医科大学学报2017，Vol.52Issue(2)：155-159,5.DOI:10.19405/j.cnki.issn1000-1492.2017.02.001
肠出血性大肠杆菌O157:H7 z0986-z1444双缺失突变株构建
Construction of z0986-z1444 double deletion mutant of enterohemorrhagic Escherichia coli O157 : H7
摘要
Abstract
Objective To construct the double deletion mutant of enterohemorrhagic Escherichia coli( EHEC) O157: H7 by knocking out z0986 gene and z1444 gene with Red recombinant system. Methods Firstly, the target frag-ments of z0986 and z1444 were established. Secondly, competent cells were struck by lightning, then the bacteria were identified through PCR. The growth curve of the ultimate positive monoclone was assayed. Results The solo deletion mutant(Δz1444/933) and the double deletion mutant(Δz0986/Δz1444/933) were successfully construc-ted and their growth curves had no significant difference compared to the wild type EHEC. Conclusion The dele-tion mutant of z0986 and z1444 has no effect on growth of EHEC. The construction of Δz1444/933 as well asΔz0986/Δz1444/933 would be helpful for further study of functions and mechanisms of z1444 gene.关键词
肠出血性大肠杆菌/Red重组系统/三型分泌系统Key words
enterohemorrhagic Escherichia coli/Red recombinant system/type Ⅲ secretion system分类
生物科学引用本文复制引用
苏莉,王建新,陈芳红,李崭,黄洁,李涛,王慧..肠出血性大肠杆菌O157:H7 z0986-z1444双缺失突变株构建[J].安徽医科大学学报,2017,52(2):155-159,5.基金项目
国家自然科学基金(编号:81401643) （编号:81401643）
国家重点基础研究计划(编号:2015CB554202) （编号:2015CB554202）
