安徽医科大学学报2017，Vol.52Issue(2)：256-260,5.DOI:10.19405/j.cnki.issn1000-1492.2017.02.024
人乳头瘤病毒与NSCLC相关性探讨
Investigation on the relevance between human papillomavirus infections and NSCLC
摘要
Abstract
Objective To investigate the relevance of human papillomavirus ( HPV) and non small cell lung cancer ( NSCLC) , and analyse the relation between the clinical pathological characteristics or a variety of genetic muta-tions of NSCLC patients and HPV infection. Methods Totally 264 cases of paraffin embedded specimens of NSCLC patients tissues were detected by immunohistochemical for HPV L1 protein,and then,109 NSCLC specimens were chosen by RT-PCR method to detect the gene sequence of HPV16/18 L1 . The adjacent epithelial tissues of primary tumor tissues and 12 cases of benign lung tissues were selected to the control group. 90 lung adenocarcino-ma( AC) specimens were tested for the genes status of EGFR,ALK,KRAS,BRAF. Results Positive rate of HPV L1 protein in NSCLC primary group was similar to that of metastases group. But the primary group positive rate was significantly higher than that in adjacent to cancer group and pulmonary benign control group. The positive rate of AC group was significantly higher than that of lung squamous cell carcinoma( SCC) group. In all 109 NSCLC carci-noma, HPV 16/18 L1 gene sequences were detected in 11 NSCLC specimens. HPV L1 protein positive patients with KRAS mutation rate was significantly higher than the HPV negative. Conclusion HPV infection may be closely related with NSCLC,especially with AC. KRAS mutations could be the driver gene type in HPV related-AC. It points out the direction of the precise treatment for lung cancer.关键词
人乳头瘤病毒/非小细胞肺癌/免疫组化/基因突变Key words
human papillomavirus/non small cell lung cancer/immunohistochemical/gene mutation分类
医药卫生引用本文复制引用
曹晓玉,操乐杰,李传应,王晓秋..人乳头瘤病毒与NSCLC相关性探讨[J].安徽医科大学学报,2017,52(2):256-260,5.基金项目
安徽省卫生厅医学科研基金(编号:13zc001) （编号:13zc001）
安徽省科技厅攻关计划项目(编号:1301042216) （编号:1301042216）
