安徽中医药大学学报2017，Vol.36Issue(1)：55-58,4.DOI:10.3969/j.issn.2095-7246.2017.01.017
异甘草酸镁对CCl4致急性肝损伤大鼠肝组织CYP1A2、CYP2E1蛋白及其mRNA表达的影响
Influence of Magnesium Isoglycyrrhizinate on Protein and mRNA Expression of CYP1A2 and CYP2E1 in Liver Tissues in Rats with Acute Liver Injury Induced by Carbon Tetrachloride
摘要
Abstract
Objective To investigate the influence of magnesium isoglycyrrhizinate on the protein and mRNA expression of CYP1A2 and CYP2E1 in liver tissues in rats with acute liver injury induced by carbon tetrachloride (CC14) and the mechanism of the liver-protecting effect of magnesium isoglycyrrhizinate from the aspect of metabolic enzymes.Methods Rats were randomly divided into normal control group,model group,and magnesium isoglycyrrhizinate group,with 6 rats in each group.All the rats were administered by caudal vein injection for 14 consecutive days;the rats in the magnesium isoglycyrrhizinate group were given magnesium isoglycyrrhizinate 20 mg/kg,and those in the normal control group and the model group were given normal saline of the same volume.The rats in the model group and the magnesium isoglycyrrhizinate group were given 25％ CC14 by gavage on day 13 at 20:00 at a dose of 1.6 mL/kg to establish a rat model of acute liver injury.At 2 hours after the last administration,the rats were sacrificed via exsanguination of the iliac artery,and the liver tissues were collected to observe pathomorphological changes of the liver by hematoxylin and eosin staining.RT-PCR and Western blot were used to measure the mRNA and protein expression of CYP1A2 and CYP2E1 in liver tissues.Results Liver pathology showed that magnesium isoglycyrrhizinate reduced liver injury.The model group had significantly higher protein and mRNA expression of CYP1A2 and CYP2E1 than the normal control group (P＜0.05),and the magnesium isoglycyrrhizinate group had significantly lower protein and mRNA expression of CYP1A2 and CYP2E1 than the model group (P＜0.05).Conclusion Magnesium isoglycyrrhizinate can reduce liver injury,and its attenuation mechanism may involve inhibiting the expression of CYP1A2 and CYP2E1.关键词
异甘草酸镁/CCl4/CYP1A2/CYP2E1/RT-PCRKey words
Magnesium isoglycyrrhizinate/Carbon tetrachloride/CYP1A2/CYP2E1/RT-PCR分类
医药卫生引用本文复制引用
陈鑫,葛芹,张彩云,陈卫东,叶茜,李鑫..异甘草酸镁对CCl4致急性肝损伤大鼠肝组织CYP1A2、CYP2E1蛋白及其mRNA表达的影响[J].安徽中医药大学学报,2017,36(1):55-58,4.基金项目
安徽省科技厅自然科学项目(1408085MH196) （1408085MH196）
国家自然科学基金项目(51303006) （51303006）
