重庆医学2017，Vol.46Issue(5)：586-589,4.DOI:10.3969/j.issn.1671-8348.2017.05.004
人牙髓干细胞和根尖乳头干细胞体外分化能力的对比研究
Comparative characterization of osteo/odontogenic differentiation capability of human dental pulp stem cells and stem cells from apical papilla in vitro
摘要
Abstract
Objective To compare the growth characteristics,proliferation and osteo/odontogenic differentiation capability of stem cells from human dental pulp (dental pulp stem cells,DPSCs) and apical papilla (stem cells from apical papilla,SCAP) in vitro.Methods Human dental pulp and apical papilla tissues were separated from impacted third molars of young healthy donors at the age of root development and digested by collagenase type Ⅰ and dispase type Ⅱ to derive primitive DPSCs and SCAP.Cells were then induced for osteo/odontongenic differentiation by medium containing β-glycerophosphate,dexamethasone and KH2PO4.Flow cytometry was utilized to test the expression of specific markers of stem cells,including CD24,CD34,CD45,CD90,CD105,CD146,STRO-1 and OCT-4.AR-S was used to display the mineralization structure and RT-PCR was applied to analyze the expression of bone sialoprotein (BSP),osteocalcin (OCN) and dentine sialophosphoprotein (DSPP).Results Both DPSCs and SCAP were positive for CD90,CD105,CD146,STRO-1 and OCT-4,in percentages varying according to cell type,without expression of CD34 or CD45.Only SCAP expressed CD24 positively.Both cells formed organized mineralization structure after 2 weeks of induction in time-dependent manner,with more mineralization by SCAP and expressed differentiation markers,including BSP,OCN and DSPP.Conclusion Human DPSCs and SCAP possess the characteristics of MSCs and could be differentiated into odontonblast-like cells in vitro.Both cells are approachable stem cell sources for dental tissue engineering.关键词
牙髓/干细胞/牙根尖/牙再矿化/组织工程Key words
dental pulp/stem cells/tooth apex/tooth remineralization/tissue engineering分类
医药卫生引用本文复制引用
谭小兵,郭宇,刘佳,徐静舒,戴青原..人牙髓干细胞和根尖乳头干细胞体外分化能力的对比研究[J].重庆医学,2017,46(5):586-589,4.基金项目
国家自然科学基金资助项目(81360161) （81360161）
云南省教育厅基金资助项目(2015Y153). （2015Y153）
