重庆医学2017，Vol.46Issue(6)：721-724,728,5.DOI:10.3969/j.issn.1671-8348.2017.06.001
鼻咽癌外泌体miR-20a通过靶向BCL2L11基因抑制肿瘤相关巨噬细胞凋亡
Exosome-derived miR-20a inhibit apoptosis of TAM by targeting BCL2L11 in nasopharyngeal carcinoma
摘要
Abstract
Objective To investigate whether exosome-derived microRNA of nasopharyngeal carcinoma suppresses apoptosis of tumor associated macrophage (TAM).Methods Target microRNAs and genes were determined by bioinformatics methods.Isolated exosomes were used to detect miR-20a expression by qRT-PCR.Furthermore,apoptosis index and proteins involved in apoptotic pathways were detected after miR-20a mimic and inhibitor transfection into macrophages.Results miR-20a expression was upregulated in isolated exosomes.miR-20a target gene was BCL2L11.MiR-20a overexpression could inhibit apoptosis of macrophages,meanwhile,apoptotic pathways related proteins Bim,caspase-9 and caspase-3 were significantly suppressed by miR-20a mimic(P＜0.05).Condusion miR-20a can suppress activation of Bim-caspase-9-casepase-3 and resulting in apoptotic inhibition of macrophages.关键词
鼻咽肿瘤/外泌体/miR-20a/BCL2L11/巨噬细胞Key words
nasopharyngeal neoplasms/exosome/miR-20a/BCL2L11/macrophages分类
医药卫生引用本文复制引用
吕龙辉,黄晓悫,熊小明,张旭,杨志惠,方红雁..鼻咽癌外泌体miR-20a通过靶向BCL2L11基因抑制肿瘤相关巨噬细胞凋亡[J].重庆医学,2017,46(6):721-724,728,5.基金项目
国家自然科学基金资助项目(81101679) （81101679）
四川省科技厅自然科学基金资助项目(14JC0178). （14JC0178）
