重庆医学2017，Vol.46Issue(6)：725-728,4.DOI:10.3969/j.issn.1671-8348.2017.06.002
HIFU联合载HSV-TK基因的纳米级超声分子探针治疗对裸鼠肝移植瘤血管生成的影响
The effect of HIFU combined with nanoscale ultrasound molecular probes with simple virus thymidine kinase gene on angiogenesis in the nude mouse tumor
摘要
Abstract
Objective To observe the change of the protein and gene expression of hypoxia inducing factor-1α(HIF-1a) and vascular endothelial growth factor (VEGF) in the nude mouse tumor,which has been treated by HIFU combined with nanoscale ultrasound molecular probes with HSV1-TK gene microvascular density.Methods Sixty nude mice were implanted with HepG2 Cells to establish subcutaneous transplanted tumor.Divided this mice into six groups at random after treated by HIFU:MB+ HSV-TK+ GPC3 (group A),MB + HSV-TK (group B),HSV-TK +GPC3 (group C),HSV-TK (group D),MB + GPC3 (group E),PBS (group F).They were injected into the tail vein every after 3 days.Mice in group A,B,D and E were exposed to ultrasound by 2 W/cm2,1 MHz,5 mintues and 0.2 mL ganciclovi(GCV) was intraperitoneally injected at the first 48 hours after injection.After the treatment,immunohistoche were used to detect the microvascular density(MVD),Western blot and immunohistoche was employed to test the protein change of the VEGF and HIF-1α,Q-PCR was used to test the mRNA gene transcription of VEGF and HIF-1α in the tumor tissues.Results After 14 days,the protein expression of HIF-1α and VEGF in group A was significantly lower than that in group B,C,D,E and F (P＜0.05),the MVD level in the tumor is also like this,and the difference is statistically significant.Conclusion Anoscale ultrasound molecular probes with HSV1-TK can reduce the the level of VEGF,MVD and HIF-1α in the tumor which has been treated by HIFU,so it can inhibit tumor growth and improve the therapeutic efficacy after HIFU treatment.关键词
血管内皮生长因子A/高强度聚焦超声/缺氧诱导因子1/分子探针/微血管密度Key words
vascular endothelial growth factor A/high-intensity focused ultrasound/hypoxia-inducible factor 1/molecular probe/microvascular density分类
医药卫生引用本文复制引用
谢辉,刘山,曾辉,李友伟,许进,丁雄,刘长安,李峰..HIFU联合载HSV-TK基因的纳米级超声分子探针治疗对裸鼠肝移植瘤血管生成的影响[J].重庆医学,2017,46(6):725-728,4.基金项目
国家自然科学基金资助项目(81272570). （81272570）
