重庆医学2017，Vol.46Issue(6)：729-731,734,4.DOI:10.3969/j.issn.1671-8348.2017.06.003
食管癌血红素加氧酶-1表达水平与氟尿嘧啶疗效的关系研究
Study on HO-1 expression in hunman esophageal carcinoma and correlates with chemotherapy sensitivity of fluorouracil
摘要
Abstract
Objective To explore the HO-1 expression levels with 5-FU chemosensitivity in esophageal squamous cell carcinoma.Methods Eca109 cells used in all experiments,MTT assay was used in cell growth curve and inhibit rate.RT-PCR and Western blot was used to detect HO-1 in cells treated with different concentrations of ZnppⅨ (0,20,80,100 μmol/L).Results The inhibitory rate of cells was significantly increased when the concentrition of ZnppⅨ increased.The inhibitory rate of cells in 80 μmol/L ZnppⅨ was higher than 20 μmol/L ZnppⅨ group(P＜0.05).The expression of HO-1 mRNA in each group was 0.50± 0.17,0.55±0.15,0.58 ± 0.09 and 0.55 ± 0.16,respectively,there was no significant difference between the two groups (P＞ 0.05).The expression of HO-1 was 0,85±0.07,0.63±0.11,0.43±0.12 and 0.25±0.10,respectively.The expression of HO-1 had significant difference (F=20.01,P＜0.01).Conclusion Eca109 cells inhibition rate positive correlated with ZnppⅨ concentrations,and ZnppⅨ were inhibited the expression of HO-1.not from gene level,but after the translation level.关键词
食管肿瘤/血红素加氧酶-1/氟尿嘧啶/药物疗法/耐受性Key words
esophageal neoplasms/heme Oxygenase-1/fluorouracil/drug therapy/tolerance分类
医药卫生引用本文复制引用
张健,马德华,陈保富,郭海谢,张波,朱成楚..食管癌血红素加氧酶-1表达水平与氟尿嘧啶疗效的关系研究[J].重庆医学,2017,46(6):729-731,734,4.基金项目
浙江省重大科技专项和优先主题项目(11C13039-2) （11C13039-2）
浙江省台州市科技局项目(11KY11). （11KY11）
